%0 Generic %A A.L., Solevåg %A C., Garcia-Hidalgo %A P.-Y., Cheung %A T.-F., Lee %A M., O'Reilly %A G.M., Schmölzer %D 2020 %T Supplementary Material for: Ventilation with 18, 21, or 100% Oxygen during Cardiopulmonary Resuscitation of Asphyxiated Piglets: A Randomized Controlled Animal Trial %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Ventilation_with_18_21_or_100_Oxygen_during_Cardiopulmonary_Resuscitation_of_Asphyxiated_Piglets_A_Randomized_Controlled_Animal_Trial/11493840 %R 10.6084/m9.figshare.11493840.v1 %2 https://karger.figshare.com/ndownloader/files/20545632 %K Animal model %K Birth asphyxia %K Inflammation %K Newborn piglet %K Newborn resuscitation %K Oxidative stress %X Background: In previous piglet experiments of profound asphyxia and cardiac arrest, recovery was similar when 21 and 100% oxygen were used for positive pressure ventilation (PPV). There was no consistent reduction in inflammation and oxidative stress in piglets ventilated with 21 or 100% oxygen. Objectives: We aimed to investigate hypoxic resuscitation, i.e., PPV with 18% oxygen, in profoundly asphyxiated piglets with cardiac arrest. We hypothesized that resuscitation with 18% oxygen would result in less inflammation and oxidative stress compared to 21 or 100% oxygen. Method: Twenty-four piglets were exposed to 30 min of normocapnic hypoxia followed by asphyxia until asystole. The piglets were randomized to PPV with 18% oxygen (n = 8), 21% oxygen (n = 8), or 100% oxygen (n = 8), and resuscitated with chest compressions and intravenous epinephrine. Return of spontaneous circulation (ROSC) was defined as an unassisted heart rate ≥100 bpm for 15 s. Lactate, GSH (total glutathione), GSSG (oxidized glutathione), and GSSG/GSH ratio were measured in myocardial and frontoparietal cortex homogenates. Interleukin (IL)-8, IL-6, IL-1β and tumor necrosis factor α were measured in frontoparietal cortex homogenates. Results: There was no difference in time to ROSC or inflammation and oxidative stress in the 3 oxygen groups. Conclusions: Resuscitation with 18% oxygen did not result in differences in inflammation and oxidative stress when compared to 21 or 100% oxygen. %I Karger Publishers