V., Nalini P.R., Deepa R., Raguraman V., Khetan M.A., Reddy S., Krishnakumar Supplementary Material for: Targeting HMGA2 in Retinoblastoma Cells in vitro Using the Aptamer Strategy High-mobility group A2 (HMGA2) protein regulates retinoblastoma (RB) cancer cell proliferation. Here, a stable phosphorothioate-modified HMGA2 aptamer was used to block HMGA2 protein function in RB cells. HMGA2-aptamer internalisation in RB cells (Y79, Weri Rb1) and non-neoplastic human retinal cells (MIO-M1) were optimised. Aptamer induced dose-dependent cytotoxicity in RB cancer cells (0.25-1.5 µM). Increased expression of <i>TGFβ</i>, <i>SMAD4</i>,<i> CDH1</i>, <i>BAX</i>, <i>CASP 3</i>,<i> PARP </i>mRNA and decreased <i>SNAI1</i>,<i> Bcl2</i> mRNA levels in aptamer-treated RB cells suggests the activation of TGFβ-<i>SMAD4</i>-mediated apoptotic pathway. Synergistic effect with etoposide was observed in aptamer treated RB cells (p value ≤0.05). No significant toxicity was observed in non-neoplastic retinal cells. •HMGA2;•HMGA2-aptamer;TGFβ-SMAD4;•Retinoblastoma;•Etoposide 2016-07-01
    https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Targeting_HMGA2_in_Retinoblastoma_Cells_in_vitro_Using_the_Aptamer_Strategy/3468911
10.6084/m9.figshare.3468911.v1