%0 Generic %A S.A., Koppes %A R., Brans %A S., Ljubojevic Hadzavdic %A M.H.W., Frings-Dresen %A T., Rustemeyer %A S., Kezic %D 2016 %T Supplementary Material for: Stratum Corneum Tape Stripping: Monitoring of Inflammatory Mediators in Atopic Dermatitis Patients Using Topical Therapy %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Stratum_Corneum_Tape_Stripping_Monitoring_of_Inflammatory_Mediators_in_Atopic_Dermatitis_Patients_Using_Topical_Therapy/3803190 %R 10.6084/m9.figshare.3803190.v1 %2 https://karger.figshare.com/ndownloader/files/5920566 %2 https://karger.figshare.com/ndownloader/files/5920569 %2 https://karger.figshare.com/ndownloader/files/5920572 %K Cytokines %K Chemokines %K Tape strips %K Dermalex %X Objective: The aim of this study was to explore the tape strip sampling technique in the assessment of stratum corneum levels of inflammatory mediators in a clinical trial setting. Methods: Thirty-eight inflammatory mediators were analyzed by a multiplex-assay in the stratum corneum, collected by adhesive tapes before and after 6 weeks of therapy, in mild and moderate atopic dermatitis (AD) patients (n = 90). Treatment was a ceramide- and magnesium-containing emollient. Results: Twenty-four mediators could quantitatively be determined. The Th2 mediators interleukin (IL)-4, IL-13, CCL2 (monocyte chemotactic protein-1), CCL22 (macrophage-derived chemokine), and CCL17 [thymus and activation-regulated chemokine (TARC)] were significantly decreased after therapy as well as IL-1β, IL-2, IL-8 (CXCL8), IL-10, acute-phase protein serum amyloid A, C-reactive protein, and vascular adhesion molecule-1. The decrease of CCL17 and IL-8 was correlated with the decrease in disease severity in a subgroup of moderate AD individuals. Conclusion: Stratum corneum tape stripping offers a minimally invasive approach for studying local levels of immunomodulatory molecules in the skin. CCL17 (TARC) and IL-8 were found to be the most promising biomarkers of AD and might be useful for investigating the course of skin diseases and the effect of local therapy. %I Karger Publishers