Supplementary Material for: In vivo Raman Microspectroscopy: Intra- and Intersubject Variability of Stratum Corneum Spectral Markers Quatela A. Miloudi L. Tfayli A. Baillet-Guffroy A. 10.6084/m9.figshare.4004937.v1 https://karger.figshare.com/articles/dataset/Supplementary_Material_for_In_vivo_Raman_Microspectroscopy_Intra-_and_Intersubject_Variability_of_Stratum_Corneum_Spectral_Markers/4004937 <strong><em>Background:</em></strong> In vivo Raman spectroscopy is a powerful tool for real-time analysis and in situ evaluation of tissues such as the skin. The efficiency of this technique has been widely demonstrated as a label-free method for in vivo evaluation of the skin. The aim of this study is to gather information about inter- and intra-individual variations in the spectral descriptors of water content and structure, organization of the lipid barrier and structure of proteins in the stratum corneum (SC). <b><i>Methods:</i></b> In vivo SC measurements were performed on 17 female volunteers aged 20-30 years (phototypes I and II). For intra-individual variability, spectral collection was performed on 5 successive days per volunteer. Shapiro-Wilk and Cochran tests were applied to check the normality and the homoscedasticity of variances. ANOVA was then applied to evaluate intra- and intergroup variability. <b><i>Results:</i></b> ANOVA was performed on the spectral descriptors of water content and structure, organization of the lipid barrier and secondary structure of proteins in the SC. No significant intra- and interday variability was observed for all volunteers. Despite the low value of the total relative standard deviation, a highly significant variation was observed between volunteers. <b><i>Conclusion:</i></b> Interindividual variability for Raman measurements is significant for a set of volunteers with normal nondiseased SC and close phototypes. This variability should be taken into consideration as a threshold for significant variance when working in vivo. 2016-10-11 13:33:50 In vivo Raman microspectroscopy Stratum corneum Interindividual variability Skin measurements Analysis of variance