Supplementary Material for: AQP5 Variants Affect Tumoral Expression of AQP5 and Survival in Patients with Early Breast Cancer
Lee S.J.
Kang B.W.
Kim J.G.
Jung J.H.
Lee J.
Kim W.W.
Park H.Y.
Jeong J.-H.
Jeong J.Y.
Park J.-Y.
Park J.H.
Chae Y.S.
10.6084/m9.figshare.4439849.v1
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_AQP5_Variants_Affect_Tumoral_Expression_of_AQP5_and_Survival_in_Patients_with_Early_Breast_Cancer/4439849
<p><b><i>Background:</i></b> Our previous study showed the association
of AQP5 upregulation with cancer proliferation and migration in breast
cancer cell lines and with unfavorable prognosis in patients with early
breast cancer (EBC). In the current study, we analyzed the association
of<i> AQP5</i> variants or their haplotypes with AQP5 expression and their prognostic impact for survival in patients with EBC. <b><i>Methods:</i></b> Three <i>AQP5</i>
polymorphisms (rs74091166, rs3736309, and rs1964676) were selected
based on the SNP database and genotyped using the Sequenom MassARRAY in
374 out of 447 patients with EBC in whom AQP5 expression had been
investigated in our previous study. <b><i>Results:</i></b> The allele
frequencies of the selected variants in the current study were similar
to those from Asian data previously reported. In a univariate analysis,
both rs74091166 and rs1964676 were statistically associated with
survival as a dominant model of minor allele. Moreover, a multivariate
survival analysis revealed that the CC genotype of rs1964676 is an
independent prognostic marker of survival in EBC patients, regardless of
stage, tumor subtype, and adjuvant treatment [hazard ratio = 0.399,
0.384, and 0.205; <i>p</i> = 0.021, 0.027, and 0.016 for disease-free
survival (DFS), distant DFS, and disease-specific survival,
respectively]. In particular, the CT/TT genotype of rs1964676 showed an
association with strong expression of AQP5 (58.6 vs. 26.0%; <i>p</i> =
0.001), without any associations with clinical or pathological
characteristics including tumor subtype, stage, or histologic grade. <b><i>Conclusion:</i></b> The current study suggests <i>AQP5 </i>rs1964676 as a new potential prognostic marker in patients with EBC involved in AQP5 expression.</p>
2016-12-15 15:46:48
Early breast cancer
Aquaporin 5
Polymorphism
Prognosis