10.6084/m9.figshare.4509413.v1 Zhu Z. Zhu Z. Yu J. Yu J. Niu Y. Niu Y. Sun S. Sun S. Liu Y. Liu Y. Saxon A. Saxon A. Zhang K. Zhang K. Li W. Li W. Supplementary Material for: Enhanced Prophylactic and Therapeutic Effects of Polylysine-Modified Ara h 2 DNA Vaccine in a Mouse Model of Peanut Allergy Karger Publishers 2017 Allergy vaccines Animal models Ara h 2 Peanut allergy Polylysine Regulatory T cells 2017-01-03 14:39:24 Figure https://karger.figshare.com/articles/figure/Supplementary_Material_for_Enhanced_Prophylactic_and_Therapeutic_Effects_of_Polylysine-Modified_Ara_h_2_DNA_Vaccine_in_a_Mouse_Model_of_Peanut_Allergy/4509413 <p><b><i>Background:</i></b> The prevalence of food allergy has been increasing, but treatment is very limited. DNA vaccination has been recognized as a promising method for the treatment of allergic diseases; however, poor immunogenicity has hindered its application. <b><i>Methods:</i></b> BALB/c mice were intradermally injected with plasmid DNA encoding the peanut protein Ara h 2 (pAra h 2) or pAra h 2 pretreated with poly-L- lysine (PLL) before or after sensitization with Ara h 2 protein. Ara h 2-specific antibodies were measured by ELISA. CD207+ dendritic cells (DCs) and Treg cells in draining lymph nodes were analyzed by flow cytometry after DNA immunization, and cytokine production in splenocytes was also analyzed. <b><i>Results:</i></b> In the prophylactic study, pretreatment with pAra h 2 or PLL-pAra h 2 resulted in lower levels of Ara h 2-specific IgG1, IgG2a, and IgE after sensitization with Ara h 2 protein, and mice in the PLL-pAra h 2 group had a significantly lower level of antibodies than those in the pAra h 2 group. In the treatment study, intradermal injection with pAra h 2 or PLL-pAra h 2 after Ara h 2 protein sensitization significantly decreased the level of Ara h 2-specific antibodies, and PLL- pAra h 2 had stronger effects than pAra h 2. There were increased numbers of CD207+ DCs and Treg cells in the mice receiving intradermal injection with PLL-pAra h 2, and splenocytes from PLL-pAra h 2-treated mice secreted increased levels of IFN-γ and IL-10. <b><i>Conclusions:</i></b> Modification of pAra h 2 with PLL improved its prophylactic and therapeutic effects in peanut-allergic mice.</p>