%0 Generic %A F.K.J., Leusink %A van Diest P.J. %A M.H., Frank %A R., Broekhuizen %A W., Braunius %A van Hooff S.R. %A S.M., Willems %A R., Koole %D 2017 %T Supplementary Material for: The Co-Expression of Kallikrein 5 and Kallikrein 7 Associates with Poor Survival in Non-HPV Oral Squamous-Cell Carcinoma %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_The_Co-Expression_of_Kallikrein_5_and_Kallikrein_7_Associates_with_Poor_Survival_in_Non-HPV_Oral_Squamous-Cell_Carcinoma/4535513 %R 10.6084/m9.figshare.4535513.v1 %2 https://karger.figshare.com/ndownloader/files/7342712 %2 https://karger.figshare.com/ndownloader/files/7342718 %2 https://karger.figshare.com/ndownloader/files/7342715 %2 https://karger.figshare.com/ndownloader/files/7342721 %2 https://karger.figshare.com/ndownloader/files/7342724 %2 https://karger.figshare.com/ndownloader/files/7342727 %2 https://karger.figshare.com/ndownloader/files/7342730 %2 https://karger.figshare.com/ndownloader/files/7342733 %2 https://karger.figshare.com/ndownloader/files/7342739 %2 https://karger.figshare.com/ndownloader/files/7342736 %K Oral squamous cell carcinoma %K Kallikrein 5 %K Kallikrein 7 %K Serine protease inhibitor Kazal-type 5 %X

Objective: Oral squamous-cell carcinoma (OSCC) still has a poor prognosis. Lymph node metastasis (LNM) is a major determinant of treatment decisions and prognosis. Serine protease inhibitor Kazal-type 5 (SPINK5) is the inhibitor of kallikrein 5 (KLK5) and KLK7. SPINK5, KLK5 and KLK7 are three of the genes of a recently validated LNM-predicting gene expression profile in OSCC. This study evaluates their clinicopathological role and value as biomarkers in OSCC. Methods: Eighty-three patients with primary OSCC, treated surgically between 1996 and 2000, were included. Gene expression data were acquired from a previously reported study. Human papillomavirus (HPV) status was determined by an algorithm for HPV-16. Protein expression for KLK5, KLK7 and SPINK5 was semi-quantitatively determined in all 83 tumours by immunohistochemistry. All expression data were correlated with clinicopathological parameters. Results: Concurrent loss of KLK5 and KLK7 correlates with worse disease-specific and overall survival (DSS and OS). Multivariate analysis proved that co-expression is an independent prognostic factor for DSS (p = 0.029) and OS (p = 0.001). Conclusion: This report demonstrates that concurrent loss of KLK5 and KLK7 associates with a poor clinical outcome in OSCC and could therefore serve as prognostic marker in this disease.

%I Karger Publishers