10.6084/m9.figshare.4585246.v1
Richters R.J.H.
Richters
R.J.H.
Falcone D.
Falcone
D.
Uzunbajakava N.E.
Uzunbajakava
N.E.
Varghese B.
Varghese
B.
Caspers P.J.
Caspers
P.J.
Puppels G.J.
Puppels
G.J.
van Erp P.E.J.
van Erp P.E.J.
van de Kerkhof P.C.M.
van de Kerkhof P.C.M.
Supplementary Material for: Sensitive Skin: Assessment of the Skin Barrier Using Confocal Raman Microspectroscopy
Karger Publishers
2017
Sensitive skin
Atopic dermatitis
Stratum corneum
Confocal Raman microspectroscopy
Transepidermal water loss
Capacitance
Skin barrier function
Skin barrier molecular composition
2017-01-25 14:53:03
Dataset
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Sensitive_Skin_Assessment_of_the_Skin_Barrier_Using_Confocal_Raman_Microspectroscopy/4585246
<p><b><i>Background/Aims:</i></b> Sensitive skin (SS), a frequently
reported condition in the Western world, has been suggested to be
underlined by an impaired skin barrier. The aim of this study was to
investigate the skin barrier molecular composition in SS subjects using
confocal Raman microspectroscopy (CRS), and to compare it with that of
non-SS (NSS) individuals as well as atopic dermatitis (AD) and allergic
rhinoconjunctivitis (AR) subjects, who frequently report SS. <b><i>Methods:</i></b> Subjects with SS (<i>n</i> = 29), NSS (<i>n</i> = 30), AD (<i>n</i> = 11), and AR (<i>n</i>
= 27) were included. Stratum corneum (SC) thickness, water,
ceramides/fatty acids, and natural moisturizing factor (NMF) were
measured by CRS along with transepidermal water loss and capacitance on
the ventral forearm, thenar, and cheek. Sebum levels were additionally
measured on the forearm and cheek. <b><i>Results:</i></b> No differences
between SS and NSS subjects were found regarding SC thickness, water,
and NMF content, yet a trend towards lower ceramides/fatty acids was
observed in the cheek. Compared to AD subjects, the SS group showed
higher ceramides/fatty acid content in the forearm, whereas no
differences emerged with AR. The correlation of macroscopic biophysical
techniques and CRS was weak,<b> </b>yet<b> </b>CRS confirmed the well-known lower content of NMF and water, and thinner SC in subjects with filaggrin mutations. <b><i>Conclusion:</i></b>
The skin barrier in SS is not impaired in terms of SC thickness, water,
NMF, and ceramides/fatty acid content. The failure of biophysical
techniques to follow alterations in the molecular composition of the
skin barrier revealed by CRS emphasizes a strong need in sensitive and
specific tools for in vivo skin barrier analysis.</p>