10.6084/m9.figshare.4602142.v1
Castro-Leyva V.
Castro-Leyva
V.
Zaga-Clavellina V.
Zaga-Clavellina
V.
Espejel-Nuñez A.
Espejel-Nuñez
A.
Vega-Sanchez R.
Vega-Sanchez
R.
Flores-Pliego A.
Flores-Pliego
A.
Reyes-Muñoz E.
Reyes-Muñoz
E.
Giono-Cerezo S.
Giono-Cerezo
S.
Nava-Salazar S.
Nava-Salazar
S.
Espino y Sosa S.
Espino y Sosa
S.
Estrada-Gutierrez G.
Estrada-Gutierrez
G.
Supplementary Material for: Decidualization Mediated by Steroid Hormones Modulates the Innate Immunity in Response to Group B Streptococcal Infection in vitro
Karger Publishers
2017
Steroid hormones
Cytokines
Chemokines
Human β-defensins
Decidual cells
Innate immunity
Pregnancy
Intrauterine infection
Group B streptococcus
Decidualization
2017-02-01 12:42:54
Figure
https://karger.figshare.com/articles/figure/Supplementary_Material_for_Decidualization_Mediated_by_Steroid_Hormones_Modulates_the_Innate_Immunity_in_Response_to_Group_B_Streptococcal_Infection_in_vitro/4602142
<p><b><i>Background:</i></b> Decidual cells play a role in the
modulation of the innate immune response to protect pregnancy against
infection. Steroid hormones regulate the innate immune response in
different tissues, and they are involved in several biological processes
like decidualization. The aim of this study was to assess if steroid
hormones modulate the innate immunity in endometrial stromal cells
(ESCs) and decidual stromal cells (DSCs) in response to group B
streptococcus (GBS) infection in vitro. <b><i>Methods:</i></b> Primary cultures of ESC were differentiated into DSC using 36 nM estradiol + 300 nM
progesterone, and both were infected with GBS overnight. Concentrations
of pro- and anti-inflammatory mediators (interleukin [IL]-1β, IL-6,
tumor necrosis factor [TNF]-α, IL-10, and TGF-β), chemokines (IL-8 and
GCP-2), and human β-defensins (HBD-1, HBD-2, and HBD-3) were measured in
the culture supernatants. <b><i>Results:</i></b> DSCs showed a significant increase in IL-6 (<i>p </i>< 0.05), TNF-α (<i>p </i>< 0.05), IL-10 (<i>p </i>< 0.01), and TGF-β (<i>p </i><
0.05) secretion after GBS infection, while these changes were not
observed in infected ESCs. IL-8 and GCP-2 increased after GBS infection,
regardless of decidualization. β-Defensins 1-3 decreased (<i>p </i>< 0.05) in ESCs after GBS infection, and hormone decidualization preserved the secretion of these antimicrobial peptides. <b><i>Conclusions:</i></b>
Decidualization mediated by steroid hormones balance the pro- and
anti-inflammatory response at the maternal-fetal interface under
infection conditions.</p>