10.6084/m9.figshare.4685449.v1
Akerman L.
Akerman
L.
Klein T.
Klein
T.
Israeli M.
Israeli
M.
Fraser A.
Fraser
A.
Sagy E.
Sagy
E.
Snast I.
Snast
I.
Mimouni D.
Mimouni
D.
David M.
David
M.
Trattner A.
Trattner
A.
Supplementary Material for: Luminex LiquiChip System for the Evaluation of Cytokine Levels in Patients with Contact Dermatitis to Potassium Dichromate
Karger Publishers
2017
Allergic contact dermatitis
Cytokines
In vitro method
Luminex LiquiChip
Peripheral blood mononuclear cells
2017-02-23 15:46:36
Journal contribution
https://karger.figshare.com/articles/journal_contribution/Supplementary_Material_for_Luminex_LiquiChip_System_for_the_Evaluation_of_Cytokine_Levels_in_Patients_with_Contact_Dermatitis_to_Potassium_Dichromate/4685449
<p><b><i>Background:</i></b> Allergic contact dermatitis (ACD) is
associated with increased production of cytokines. The patch test is the
“gold-standard” diagnostic method, but it poses a risk of false
results. <b><i>Objective:</i></b> To evaluate a novel laboratory
technique, the Luminex LiquiChip, which simultaneously measures blood
levels of multiple cytokines, as a diagnostic tool in patients with
chrome-induced ACD. <b><i>Methods:</i></b> The study group included 20
patients with ACD and relevant patch test results for potassium
dichromate and 19 patients with ACD for nickel or fragrance as control.
Peripheral blood mononuclear cells (PBMCs) were cultured in the presence
and absence of potassium dichromate. The Luminex LiquiChip was used to
measure levels of the following cytokines: granulocyte-macrophage
colony-stimulating factor, interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6,
IL-8, IL-10, interferon-γ, and tumor necrosis factor (TNF)-α. <b><i>Results:</i></b>
Potassium dichromate-stimulated PBMCs secreted significantly higher
amounts of all cytokines except TNF-α than nonstimulated PBMCs. PBMCs
from patients with ACD to chromium secreted significantly higher amounts
of all cytokines tested, except IL-4, compared to PBMCs from patients
with ACD to nickel or fragrance. <b><i>Conclusions:</i></b> Potassium
dichromate stimulates the production of both Th1- and Th2-type cytokines
in patients with chrome allergy. The Luminex LiquiChip is a promising
in vitro method and may serve as a diagnostic tool for ACD.</p>