PowerPoint Slides for: Rapamycin Enhances Repressed Autophagy and Attenuates Aggressive Progression in a Rat Model of IgA Nephropathy
Liu D.
Liu Y.
Chen G.
He L.
Tang C.
Wang C.
Yang D.
Li H.
Dong Z.
Liu H.
10.6084/m9.figshare.4702639.v1
https://karger.figshare.com/articles/figure/PowerPoint_Slides_for_Rapamycin_Enhances_Repressed_Autophagy_and_Attenuates_Aggressive_Progression_in_a_Rat_Model_of_IgA_Nephropathy/4702639
<p><b><i>Background:</i></b> IgA nephropathy (IgAN) has been considered
to be the most frequent form of primary glomerulonephritis that occurs
worldwide with a variety of factors involved in its occurrence and
development. The impact of autophagy in IgAN, however, remains partially
unclear. This study was designed to investigate the effects of
rapamycin in an IgAN model. <b><i>Method:</i></b> After establishing an
IgAN rat model, SD rats were divided into 4 groups: control, control +
rapamycin, IgAN, IgAN + rapamycin. Proteinuria and the pathological
changes and the level of autophagy of kidney were texted. Identify the
expression of phosphorylation and total mammalian target of rapamycin
(mTOR) and s6k1 as well as cyclin D1 in the kidney of rats through
Western blot and immunohistochemistry. <b><i>Results:</i></b> With
rapamycin treatment, we observed a significant reduction in the
progression of proteinuria as well as alleviation of pathological
lesions in IgAN rats. Besides, autophagy was inhibited, while the
mTOR/S6k1 pathway was activated and expression of cyclin D1 was
increased in IgAN. Rapamycin treatment increased autophagy and decreased
the expression of cyclin D1. <b><i>Conclusion:</i></b> These results may suggest that mTOR-mediated autophagy inhibition may result in mesangial cell proliferation in IgAN.</p>
2017-02-28 14:41:54
IgA nephropathy
Autophagy
Rapamycin
Mamalian target of rapamycin
Cell cycle
Cell proliferation