Supplementary Material for: c-Rel Promotes Invasion of Choriocarcinoma Cells via PI3K/AKT Signaling
Sekiya Y.
Yamamoto E.
Niimi K.
Nishino K.
Nakamura K.
Kotani T.
Kajiyama H.
Shibata K.
Kikkawa F.
10.6084/m9.figshare.4721014.v1
https://karger.figshare.com/articles/figure/Supplementary_Material_for_c-Rel_Promotes_Invasion_of_Choriocarcinoma_Cells_via_PI3K_AKT_Signaling/4721014
<p><b><i>Objective:</i></b> Choriocarcinoma is the most common
epithelial cancer among gestational trophoblastic diseases (GTDs); the
mechanism of trophoblastic carcinogenesis is unknown. This study aimed
to examine the expression of NF-κB family proteins in GTDs and placental
tissues as well as the roles of c-Rel in choriocarcinoma. <b><i>Methods:</i></b>
We examined the expression of NF-κB family proteins in normal placenta
and hydatidiform mole tissues as well as extravillous trophoblast (EVT)
and choriocarcinoma cell lines by Western blot and immunohistochemistry.
Immunoprecipitation was performed to determine which proteins can bind
with c-Rel in choriocarcinoma cells. To investigate the roles of c-Rel
in choriocarcinoma, we examined the effects of c-Rel knockdown and
overexpression on cell proliferation, migration, and invasion using
small interfering RNAs and gene activation plasmid. <b><i>Results:</i></b>
The expression of c-Rel was strong in choriocarcinoma and EVTs, but
very weak in villi of normal placenta and hydatidiform mole.
Immunoprecipitation suggested that c-Rel heterodimerizes with p65 in
choriocarcinoma. c-Rel knockdown reduced invasion, migration, and AKT
phosphorylation in choriocarcinoma cells. c-Rel overexpression in
choriocarcinoma increased migratory and invasive abilities, and the
effect on invasion was inhibited by a PI3K inhibitor. <b><i>Conclusion:</i></b>
These findings suggest that c-Rel might play a role in promoting the
invasion of choriocarcinoma cells through PI3K/AKT signaling.</p>
2017-03-03 09:44:55
Choriocarcinoma
c-related
Invasion
Migration
p65