Supplementary Material for: A Non-Human Primate BAC Resource to Study Interchromosomal Segmental Duplications
Kirsch S.
Hodler C.
Schempp W.
10.6084/m9.figshare.4781140.v1
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_A_Non-Human_Primate_BAC_Resource_to_Study_Interchromosomal_Segmental_Duplications/4781140
<p>Segmental duplications (SDs) are involved in the reshaping and
evolutionary development of primate genome architecture. Their intrinsic
property to promote genomic instability facilitates genome
rearrangements, thereby contributing to karyotype diversity in primates.
However, comparative analyses of SDs based on whole-genome shotgun
assemblies of primate genomes may lead to a distorted view of their
evolutionary dynamics as this method will incorrectly assemble or simply
not represent these regions. Therefore high-quality sequences of
chromosomally assigned SDs are indispensable for unraveling the
amplification and dispersal pattern of SDs during primate evolution.
Here, we use an updated version of the ancestral duplicon state of the
non-palindromic SDs of all 4 human Y-chromosome
euchromatin/heterochromatin transition regions to perform a survey of
duplicons genome-wide across 7 primate species. By adjusting
experimental conditions to the mean nucleotide sequence divergence to
human we identified 11,075 BAC clones carrying primate orthologs or
paralogs of human Y chromosome-derived duplicons. Preliminary results
indicate lineage-specific amplification of duplicons in prosimians and
gibbons. This BAC-based framework represents the first complete set of a
defined number of duplicons over 60 million years of primate evolution.
Comparative sequence analysis of this genetic resource can contribute
to our deeper understanding of the impact of segmental duplications on
primate genome evolution.</p>
2017-03-23 15:04:12
Evolution
Primates
Segmental duplication
Y chromosome