10.6084/m9.figshare.4884494.v1
Xiao H.
Xiao
H.
Deng M.
Deng
M.
Yang B.
Yang
B.
Hu Z.
Hu
Z.
Tang J.
Tang
J.
Supplementary Material for: Pretreatment with 17β-Estradiol Attenuates Cerebral Ischemia-Induced Blood-Brain Barrier Disruption in Aged Rats: Involvement of Antioxidant Signaling
Karger Publishers
2017
Stroke
Aging
Estradiol
Oxidative stress
Blood-brain barrier
2017-04-18 12:09:40
Journal contribution
https://karger.figshare.com/articles/journal_contribution/Supplementary_Material_for_Pretreatment_with_17_-Estradiol_Attenuates_Cerebral_Ischemia-Induced_Blood-Brain_Barrier_Disruption_in_Aged_Rats_Involvement_of_Antioxidant_Signaling/4884494
<p><b><i>Background/Aims:</i></b> Blood-brain barrier (BBB) disruption
is often induced in brain injury and particularly associated with
cerebral ischemia in stroke. Estradiol (17β-estradiol 3-benzoate, E2),
an endogenous steroid hormone, has been reported to play a protective
role against cerebrovascular pathologies. Here we aimed to determine the
potential effects of E2 on the integrity of BBB and brain damage
following middle cerebral artery occlusion (MCAO). <b><i>Methods:</i></b>
Aged (24- month-old) ovariectomized female rats were administered E2
for 2 months through daily intraperitoneal injections prior to induction
of MCAO. <b><i>Results:</i></b> Compared to vehicle controls, E2 had a
dose-dependent effect in reducing the severity of brain injuries while
maintaining the integrity of the BBB in aged rats. The neuroprotective
effects of E2 were associated with the rescued tight junction loss,
decreased oxidative stress, and attenuated ERK activation in the
ischemic brains. <b><i>Conclusion:</i></b> Our data suggest a beneficial
role of E2 in aged stroke patients, associated with a protective effect
of E2 against BBB disruption in aging-related brain ischemia.</p>