Supplementary Material for: The Aachen Minipig: Phenotype, Genotype, Hematological and Biochemical Characterization, and Comparison to the Göttingen Minipig
Pawlowsky K.
Ernst L.
Steitz J.
Stopinski T.
Kögel B.
Henger A.
Kluge R.
Tolba R.
10.6084/m9.figshare.4898108.v1
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_The_Aachen_Minipig_Phenotype_Genotype_Hematological_and_Biochemical_Characterization_and_Comparison_to_the_G_ttingen_Minipig/4898108
<p><b><i>Background:</i></b> The pig is one of the most frequently used
large animal models for biomedical research, especially in the field of
translational research and surgical models. While standard livestock
breeds are used in short-term and acute studies, minipig breeds are the
preferred breeds in long-term and chronic studies due to their limited
growth and body weight. <b><i>Objective:</i></b> In consideration of the
3R principle (refinement, reduction, replacement) and the increasing
demand, the aim of this study was to generate a new, robust,
non-specific-pathogen-free minipig breed, the Aachen minipig. <b><i>Methods:</i></b>
Phenotype, genotype, and hematological as well as clinical chemistry
parameters were characterized, and reference values of the Aachen
minipig were generated and compared to the values in the commonly used
Göttingen minipig. Organ weights of the heart, kidney, liver, lung,
spleen, and brain were determined using a laboratory balance. Blood
samples were collected for hematology and clinical chemistry. Assessment
of genetic diversity was performed by microsatellite markers. Nasal
swabs were collected from 11 individual minipigs representing 6 races
for DNA extraction. DNA was quantified and the identity and origin of
the Aachen minipigs at the genomic level was determined by
microsatellites. <b><i>Results:</i></b> The Aachen minipig established
here is based on the Mini-LEWE breed and consists of the Vietnamese
potbelly pig, the Schwäbisch Hällisch Landpig, the German Landrace, and
the Minnesota minipig. Relative organ weights (lung, heart, kidneys,
brain), hematology (hemoglobin, hematocrit, platelet count, mean
corpuscular hemoglobin concentration, segmented neutrophils,
lymphocytes, eosinophils, basophils), and clinical chemistry parameters
(sodium, calcium, chloride, alkaline phosphatase, aspartate
aminotransferase, alanine aminotransferase, gamma-glutamyl transferase,
lactate dehydrogenase, triglycerides, blood urea nitrogen, creatinine,
total bilirubin, total protein, creatine kinase) of the Aachen minipigs
and the Göttingen minipigs were not significantly different. Significant
differences where only seen in relative organ weights (liver, spleen),
hematology (red blood cell count, mean corpuscular volume, mean
corpuscular hemoglobin, white blood cell count, banded neutrophils,
monocytes), and clinical chemistry parameters (inorganic phosphorus,
potassium, glucose, cholesterol, albumin, amylase). <b><i>Conclusion:</i></b>
The Aachen minipig is a suitable model for research due to its
similarity to other minipig breeds, especially the Göttingen minipig.
The reference values established in this study may be used for the
comparison of scientific data and encourage the use of the Aachen
minipig as an animal model for biomedical research.</p>
2017-04-21 07:47:41
Aachen minipig
Clinical chemistry
Genotype
Hematology
Reference values