Supplementary Material for: Comparison of the Performance of Skin Prick, ImmunoCAP, and ISAC Tests in the Diagnosis of Patients with Allergy
Griffiths R.L.M.
El-Shanawany T.
Jolles S.R.A.
Selwood C.
Heaps A.G.
Carne E.M.
Williams P.E.
10.6084/m9.figshare.4929728.v1
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Comparison_of_the_Performance_of_Skin_Prick_ImmunoCAP_and_ISAC_Tests_in_the_Diagnosis_of_Patients_with_Allergy/4929728
<p><b><i>Background:</i></b> Allergy is diagnosed from typical symptoms,
and tests are performed to incriminate the suspected precipitant. Skin
prick tests (SPTs) are commonly performed, inexpensive, and give
immediate results. Laboratory tests (ImmunoCAP) for serum
allergen-specific IgE antibodies are usually performed more selectively.
The immuno-solid phase allergen chip (ISAC) enables testing for
specific IgE against multiple allergen components in a multiplex assay. <b><i>Methods:</i></b>
We retrospectively analysed clinic letters, case notes, and laboratory
results of 118 patients attending the National Adult Allergy Service at
the University Hospital of Wales who presented diagnostic difficulty, to
evaluate which testing strategy (SPT, ImmunoCAP, or ISAC) was the most
appropriate to use to confirm the diagnosis in these complex patients,
evaluated in a “real-life” clinical service setting. <b><i>Results:</i></b>
In patients with nut allergy, the detection rates of SPTs (56%) and
ISAC (65%) were lower than those of ImmunoCAP (71%). ISAC had a higher
detection rate (88%) than ImmunoCAP (69%) or SPT (33%) in the diagnosis
of oral allergy syndrome. ImmunoCAP test results identified all 9
patients with anaphylaxis due to wheat allergy (100%), whereas ISAC was
positive in only 6 of these 9 (67%). <b><i>Conclusions:</i></b> In this
difficult diagnostic group, the ImmunoCAP test should be the preferred
single test for possible allergy to nuts, wheat, other specific foods,
and anaphylaxis of any cause. In these conditions, SPT and ISAC tests
give comparable results. The most useful single test for oral allergy
syndrome is ISAC, and SPT should be the preferred test for latex
allergy.</p>
2017-04-28 10:12:23
Allergy diagnosis
Sensitivity
Specificity
Skin prick tests
Allergen-specific IgE (ImmunoCAP) tests
ISAC allergen array
Component-resolved diagnostics