%0 Generic %A R., Cassata %A A., Iannuzzi %A P., Parma %A De Lorenzi L. %A V., Peretti %A A., Perucatti %A L., Iannuzzi %A Di Meo G.P. %D 2008 %T Supplementary Material for: Clinical, cytogenetic and molecular evaluation in a dog with bilateral cryptorchidism and hypospadias %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Clinical_cytogenetic_and_molecular_evaluation_in_a_dog_with_bilateral_cryptorchidism_and_hypospadias/5120365 %R 10.6084/m9.figshare.5120365.v1 %2 https://karger.figshare.com/ndownloader/files/8704279 %K Medicine %X The aim of this study was to estimate prognostic factors in a Dalmatian dog with bilateral cryptorchidism and hypospadias. Cytogenetic and molecular analyses revealed a normal karyotype (2n = 78,XY) and the presence of SRY, INSL3 and RXFP2 genes with a normal DNA sequence for SRY and RXFP2, while the INSL3 sequence differed slightly from the normal one due to a heterozygous nucleotide change involving amino acid 22 of the INSL3 dog precursor protein. Levels of plasmatic testosterone were only 0.01 ng/ml, while FSH and LH serum levels were not detectable. After the human chorionic gonadotropin (hCG) test, the serum testosterone level was 0.01 ng/ml. Therefore, the phenotypic aetiology of this subject can not be well-defined because cryptorchidism and hypospadias were frequent clinical features with high genetic heterogeneity. %I Karger Publishers