K.H., Yuill A.J., McNeish Y., Kansui C.J., Garland K.A., Dora Supplementary Material for: Nitric Oxide Suppresses Cerebral Vasomotion by sGC-Independent Effects on Ryanodine Receptors and Voltage-Gated Calcium Channels <i>Background/Aims:</i> In cerebral arteries, nitric oxide (NO) release plays a key role in suppressing vasomotion. Our aim was to establish the pathways affected by NO in rat middle cerebral arteries. <i>Methods:</i> In isolated segments of artery, isometric tension and simultaneous measurements of either smooth muscle membrane potential or intracellular [Ca<sup>2+</sup>] ([Ca<sup>2+</sup>]<sub>SMC</sub>) changes were recorded. <i>Results:</i> In the absence of <i>L</i>-NAME, asynchronous propagating Ca<sup>2+</sup> waves were recorded that were sensitive to block with ryanodine, but not nifedipine. <i>L</i>-NAME stimulated pronounced vasomotion and synchronous Ca<sup>2+</sup> oscillations with close temporal coupling between membrane potential, tone and [Ca<sup>2+</sup>]<sub>SMC</sub>. If nifedipine was applied together with <i>L</i>-NAME, [Ca<sup>2+</sup>]<sub>SMC</sub> decreased and synchronous Ca<sup>2+</sup> oscillations were lost, but asynchronous propagating Ca<sup>2+</sup> waves persisted. Vasomotion was similarly evoked by either iberiotoxin, or by ryanodine, and to a lesser extent by ODQ. Exogenous application of NONOate stimulated endothelium-independent hyperpolarization and relaxation of either <i>L</i>-NAME-induced or spontaneous arterial tone. NO-evoked hyperpolarization involved activation of BK<sub>Ca</sub> channels via ryanodine receptors (RYRs), with little involvement of sGC. Further, in whole cell mode, NO inhibited current through L-type voltage-gated Ca<sup>2+</sup> channels (VGCC), which was independent of both voltage and sGC. <i>Conclusion:</i> NO exerts sGC-independent actions at RYRs and at VGCC, both of which normally suppress cerebral artery myogenic tone. Nitric oxide;Membrane potential;Calcium signalling;Vascular smooth muscle;Cerebral arteries;Vasomotion 2009-09-04
    https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Nitric_Oxide_Suppresses_Cerebral_Vasomotion_by_sGC-Independent_Effects_on_Ryanodine_Receptors_and_Voltage-Gated_Calcium_Channels/5120812
10.6084/m9.figshare.5120812.v1