Supplementary Material for: The CD6 Scavenger Receptor Is Differentially Expressed on a CD56<sup>dim</sup> Natural Killer Cell Subpopulation and Contributes to Natural Killer-Derived Cytokine and Chemokine Secretion Braun M. Müller B. ter Meer D. Raffegerst S. Simm B. Wilde S. Spranger S. Ellwart J. Mosetter B. Umansky L. 10.6084/m9.figshare.5121589.v1 https://karger.figshare.com/articles/dataset/Supplementary_Material_for_The_CD6_Scavenger_Receptor_Is_Differentially_Expressed_on_a_CD56_sup_dim_sup_Natural_Killer_Cell_Subpopulation_and_Contributes_to_Natural_Killer-Derived_Cytokine_and_Chemokine_Secretion/5121589 The CD6 scavenger receptor is known to be expressed on virtually all T cells and is supposed to be involved in costimulation, synapse formation, thymic selection and leukocyte migration. Here, we demonstrate that CD6 is differentially expressed by a subpopulation of peripheral CD56<sup>dim</sup> natu- ral killer (NK) cells and absent on CD56<sup>bright</sup> NK cells. CD56<sup>dim</sup>CD16<sup>+</sup> cells represent the major NK subset in the periphery, and most cells within this group are positive for CD6. Most killer immunoglobulin-like receptor- and immunoglobulin-like transcript-positive cells also belong to the CD6<sup>+</sup> subpopulation, as expected from their restricted expression on CD56<sup>dim</sup> NK cells. In addition, CD6<sup>+</sup> NK cells are similar to the newly identified CD94<sup>low</sup>CD56<sup>dim</sup> NK subpopulation and most distant from the recently defined CD27<sup>+</sup> NK subpopulation based on the reverse correlation of expression between CD6 and CD27, a marker associated primarily with CD56<sup>bright</sup> NK cells. With respect to CD6 function on NK cells, direct CD6 triggering did not result in degranulation but induced secretion of cytokines (interferon-γ and tumor necrosis factor-α) and chemokines [CXCL10 (IP-10), CXCL1 (GRO-α)]. Thus, CD6 expression on peripheral NK cells marks a novel CD56<sup>dim</sup> subpopulation associated with distinct patterns of cytokine and chemokine secretion. 2010-12-18 00:00:00 CD6 Chemokines Cytokines Natural killer cells Natural killer subpopulations Scavenger receptor