10.6084/m9.figshare.5123308.v1 Iwamoto A. Iwamoto A. Osawa A. Osawa A. Kawai M. Kawai M. Honda H. Honda H. Yoshida S. Yoshida S. Furuya N. Furuya N. Kato J. Kato J. Supplementary Material for: Mutations in the Essential <i>Escherichia coli </i>Gene, <i>yqgF</i>, and Their Effects on Transcription Karger Publishers 2012 Escherichia coli Essential genes NusA Rho Transcription termination 2012-02-21 00:00:00 Dataset https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Mutations_in_the_Essential_i_Escherichia_coli_i_Gene_i_yqgF_i_and_Their_Effects_on_Transcription/5123308 The <i>Escherichia coli</i> <i>yqgF</i> gene is highly conserved across a broad spectrum of bacterial genomes. The gene was first identified as being essential for cell growth during screening for targets for broad-spectrum antibiotics. YqgF is structurally similar to RuvC, a Holliday junction resolvase, but its function has not been established. This study describes the isolation of a temperature-sensitive <i>yqgF</i> mutant, the growth of which was inhibited by <i>rho</i> or <i>nusA</i> multicopy plasmids, indicating that YqgF is involved in transcription. Rho is a global transcription termination factor that acts at Rho-dependent terminator sites, which exist not only at the ends of genes but also within genes. The transcription of genes possessing intragenic, or upstream, Rho-dependent terminators was reduced in temperature-sensitive <i>yqgF</i> mutants. This transcription inhibition was sensitive to the Rho inhibitor, bicyclomycin. In addition, the transcription of mutant <i>tnaA</i> genes defective for upstream Rho-dependent termination was not significantly affected by the <i>yqgF</i> mutation. Taken together, these results suggest that YqgF is involved in anti-termination at Rho-dependent terminators in vivo.