%0 Generic %A M., Lopo %A A., Montagud %A E., Navarro %A I., Cunha %A A., Zille %A de Córdoba P.F. %A P., Moradas-Ferreira %A P., Tamagnini %A J.F., Urchueguía %D 2012 %T Supplementary Material for: Experimental and Modeling Analysis of Synechocystis sp. PCC 6803 Growth %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Experimental_and_Modeling_Analysis_of_i_Synechocystis_i_sp_PCC_6803_Growth/5123338 %R 10.6084/m9.figshare.5123338.v1 %2 https://karger.figshare.com/ndownloader/files/8709064 %K Cyanobacteria %K Dynamic flux balance analysis %K Growth %K pH %K Synechocystis %X Background/Aims: The influence of different parameters such as temperature, irradiance, nitrate concentration, pH, and an external carbon source on Synechocystis PCC 6803 growth was evaluated. Methods: 4.5-ml cuvettes containing 2 ml of culture, a high-throughput system equivalent to batch cultures, were used with gas exchange ensured by the use of a Parafilm™ cover. The effect of the different variables on maximum growth was assessed by a multi-way statistical analysis. Results: Temperature and pH were identified as the key factors. It was observed that Synechocystis cells have a strong influence on the external pH. The optimal growth temperature was 33°C while light-saturating conditions were reached at 40 µE·m–2·s–1. Conclusion: It was demonstrated that Synechocystis exhibits a marked difference in behavior between autotrophic and glucose-based mixotrophic conditions, and that nitrate concentrations did not have a significant influence, probably due to endogenous nitrogen reserves. Furthermore, a dynamic metabolic model of Synechocystis photosynthesis was developed to gain insights on the underlying mechanism enabling this cyanobacterium to control the levels of external pH. The model showed a coupled effect between the increase of the pH and ATP production which in turn allows a higher carbon fixation rate. %I Karger Publishers