%0 Generic %A R., Murgas Torrazza %A N., Li %A C., Young %A F., Kobeissy %A M., Chow %A S., Chen %A V., Mai %A R., Sharma %A M., Hudak %A J., Shuster %D 2013 %T Supplementary Material for: Pilot Study Using Proteomics to Identify Predictive Biomarkers of Necrotizing Enterocolitis from Buccal Swabs in Very Low Birth Weight Infants %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Pilot_Study_Using_Proteomics_to_Identify_Predictive_Biomarkers_of_Necrotizing_Enterocolitis_from_Buccal_Swabs_in_Very_Low_Birth_Weight_Infants/5125381 %R 10.6084/m9.figshare.5125381.v1 %2 https://karger.figshare.com/ndownloader/files/8712283 %K Necrotizing enterocolitis %K Biomarker %K Very low birth weight infants %K Proteomics %K Systems biology %K Prediction %K Diagnosis %X Background: Necrotizing enterocolitis (NEC) is a major cause of death and morbidity in very low birth weight infants. Objective: To identify biomarker(s) that would predict NEC using buccal swab samples utilizing a proteomic approach. Methods: Cumulative buccal swab samples derived from very low birth weight preterm infants (<32 weeks' gestational age and <1,250 g) at 1, 2 and 3 weeks prior to the development of NEC and matched controls were subjected to two-dimensional difference gel electrophoresis and LC-MS/MS analysis for proteomic protein discovery. After identification of 21 altered proteins, we chose 3 candidate proteins using a broad systems biologic analysis approach that suggested several altered cellular processes that could be associated with NEC. Results: Preliminary validation studies using Western blots on these samples and 10 additional NEC and 10 matched control buccal samples collected within 2 or 3 weeks before NEC diagnosis analysis showed lower interleukin-1 receptor antagonist. Conclusion: Our results suggest that interleukin-1 receptor antagonist is worthy of further studies to determine its utility in helping predict NEC. %I Karger Publishers