%0 Generic
%A R., Rodríguez-Pérez
%A R.I., Monsalve
%A A., Galán
%A T., Perez-Piñar
%A A., Umpierrez
%A M., Lluch-Bernal
%A F., Polo
%A M.L., Caballero
%D 2014
%T Supplementary Material for: Cross-Reactivity between Anisakis spp. and Wasp Venom Allergens
%U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Cross-Reactivity_between_b_i_Anisakis_i_b_spp_and_Wasp_Venom_Allergens/5126104
%R 10.6084/m9.figshare.5126104.v1
%2 https://karger.figshare.com/ndownloader/files/8713324
%2 https://karger.figshare.com/ndownloader/files/8713327
%2 https://karger.figshare.com/ndownloader/files/8713330
%K Anisakis allergens
%K Anisakis allergy
%K Anisakis simplex
%K Component-resolved diagnosis
%K Hymenoptera
%K Wasp venom allergy
%X Background: Anisakiasis is caused by the consumption of raw or undercooked fish or cephalopods parasitized by live L3 larvae of nematode Anisakis spp. Larvae anchor to stomach mucosa releasing excretion/secretion products which contain the main allergens. It has been described that nematode larvae release venom allergen-like proteins among their excretion/secretion products. We investigated potential cross-reactivity between Anisakis and wasp venom allergens. Methods: Two groups of 25 patients each were studied: wasp venom- and Anisakis-allergic patients. Sera from patients were tested by ImmunoCAP, dot-blotting with recombinant Anisakis allergens and ADVIA-Centaur system with Hymenoptera allergens. Cross-reactivity was assessed by IgE immunoblotting inhibition assays. Role of cross-reactive carbohydrate determinants (CCDs) was studied by inhibition with bromelain and periodate treatment. Results: A total of 40% of wasp venom-allergic patients had specific IgE to Anisakis simplex and 20% detected at least one of the Anisakis recombinant allergens tested. Likewise, 44% of Anisakis-allergic patients had specific IgE to Vespula spp. venom and 16% detected at least one of the Hymenoptera allergens tested. Wasp venom-allergic patients detected CCDs in Anisakis extract and peptide epitopes on Anisakis allergens rAni s 1 and rAni s 9, whereas Anisakis-allergic patients only detected CCDs on nVes v 1 allergen from Vespula spp. venom. The only Anisakis allergen inhibited by Vespula venom was rAni s 9. Conclusions: This is the first time that cross-sensitization between wasp venom and Anisakis is described. CCDs are involved in both cases; however, peptide epitopes are only recognized by wasp venom-allergic patients.
%I Karger Publishers