Supplementary Material for: Evaluating Dissociated Optic Nerve Fiber Layer Appearance Using En Face Layer Imaging Produced by Optical Coherence Tomography S.Kusuhara W.Matsumiya H.Imai S.Honda Y.Tsukahara A.Negi 2014 <b><i>Purpose:</i></b> To investigate the incidence of and risk factors for a dissociated optic nerve fiber layer (DONFL) appearance after pars plana vitrectomy (PPV). <b><i>Methods:</i></b> We retrospectively reviewed 189 eyes that underwent PPV with internal limiting membrane removal and judged the presence/absence of an apparent DONFL based on en face layer images produced by spectral-domain optical coherence tomography (SD-OCT). <b><i>Results:</i></b> An apparent DONFL was observed in 47 (24.9%) eyes. The incidence of an apparent DONFL was significantly higher in the macular hole (MH) group (76.5%) than in the non-MH group (epiretinal membrane, diabetic macular edema, retinal vein occlusion, and others; 4.9%; p < 0.001). In the logistic regression analysis, surgical indication for MH was identified as the most significant DONFL risk factor (odds ratio 63.7; p = 1.05 × 10<sup>-8</sup>). <b><i>Conclusion:</i></b> Postoperative OCT en face layer imaging clarified that MH eyes are liable to have an apparent DONFL following PPV.