10.6084/m9.figshare.5127268.v1 Ono M. Ono M. Sonoyama W. Sonoyama W. Yamamoto K. Yamamoto K. Oida Y. Oida Y. Akiyama K. Akiyama K. Shinkawa S. Shinkawa S. Nakajima R. Nakajima R. Pham H.T. Pham H.T. Hara E.S. Hara E.S. Kuboki T. Kuboki T. Supplementary Material for: Efficient Bone Formation in a Swine Socket Lift Model Using <b><i>Escherichia coli-</i></b>Derived Recombinant Human Bone Morphogenetic Protein-2 Adsorbed in β-Tricalcium Phosphate Karger Publishers 2015 Escherichia coli-derived recombinant human bone morphogenetic protein-2 β-Tricalcium phosphate Bone-to-implant contact Bone density Bone regeneration Sinus lift Implant Biomaterials Surgery 2015-01-22 00:00:00 Dataset https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Efficient_Bone_Formation_in_a_Swine_Socket_Lift_Model_Using_b_i_Escherichia_coli-_i_b_Derived_Recombinant_Human_Bone_Morphogenetic_Protein-2_Adsorbed_in_-Tricalcium_Phosphate/5127268 Several preclinical studies have shown that <i>Escherichia coli-</i>derived bone morphogenetic protein-2 (E-BMP-2) is as effective as mammalian cell-derived bone morphogenetic protein-2 (C-BMP-2) in the treatment of bone defects. However, further investigation of the effectiveness and determination of the optimal dosage of E-BMP-2 in large animals are still necessary before its full application in humans. This study investigated the efficiency of different concentrations of E-BMP-2 adsorbed in β-TCP for bone augmentation and osseointegration of immediate dental implants in a swine socket lift model. Following exposure of the maxillary sinus lateral wall, a 3.4-mm (diameter) cavity was drilled and filled with 0.1 g of β-TCP containing different doses of E-BMP-2 (0, 10, 30, or 100 μg/site) to lift the Schneiderian membrane. A dental implant was then immediately inserted. Bone-to-implant contact (BIC) and bone density (BD) examined via histological analysis were used as parameters to assess E-BMP-2 efficiency in bone formation. The implant stability quotient (ISQ) was measured using Osstell to determine the effect of E-BMP-2/β-TCP on implant stability. After 8 weeks, the groups that received 30 and 100 μg of E-BMP-2 showed substantial new bone formation in the elevated space, while no bone formation was observed with β-TCP alone. Accordingly, BIC and BD presented a dose-dependent response to increasing doses of E-BMP-2. However, there was no increase in implant stability with E-BMP-2 treatment. In conclusion, the E-BMP-2/β-TCP combination was efficient in bone formation and osseointegration of dental implants in a socket lift model in mini-pigs.