Y.-L., Chien C.-Y., Lin K.-L., Lo Y.-M., Cheng Supplementary Material for: Development and Mapping of CL-Repeat Display Markers on the Maize B Chromosome The CL-repeat is a repetitive sequence that is unique to the maize B chromosome, where it resides in the centromeric knob and the first 3 distal heterochromatic regions of the long arm. Given this organization, it would be desirable to identify molecular markers that are specifically distributed in the B chromosome. In this report, the CL-repeat has been used to develop a class of molecular markers for the maize B chromosome. To this end, a modified transposon display procedure designated as CL-repeat display was used to generate and display 26 genomic fragments that are specific to the B chromosome, all of which were cloned and sequenced. The sequences of 19 fragments were highly homologous to the 5′ or 3′ terminus of the CL-repeat. Five of these fragments also contained sequences that were homologous to sequences of the B chromosome centromere. Four of the other 7 fragments shared homology with B chromosome centromere sequences, and the remaining 3 were of unidentified sequences. Using 13 B-10L translocations with various breakpoints along the B chromosome long arm, the 26 CL-repeat display markers were mapped to definite regions of the B chromosome. This strategy should be feasible for the development of molecular markers for the B chromosome in maize and in other species where B chromosome-specific repeats have been identified. B chromosome;B-10L translocation;CL-repeat;FISH;Maize 2015-01-20
    https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Development_and_Mapping_of_CL-Repeat_Display_Markers_on_the_Maize_B_Chromosome/5127460
10.6084/m9.figshare.5127460.v1