Y., Hibino N., Sakamoto Y., Naito K., Goto H.Z., Oo K., Sentani T., Hinoi H., Ohdan N., Oue W., Yasui Supplementary Material for: Significance of miR-148a in Colorectal Neoplasia: Downregulation of miR-148a Contributes to the Carcinogenesis and Cell Invasion of Colorectal Cancer <b><i>Objective:</i></b> Colorectal cancer (CRC) develops through the deregulation of gene expression and the accumulation of epigenetic abnormalities, leading to tumor cell acquisition of malignant features. MicroRNAs (miRNAs) play a critical role in cancer development, where they can act as oncogenes or oncosuppressors. <b><i>Methods:</i></b> miR-148a expression was measured by qRT-PCR in patients with colorectal adenoma (n = 21) and CRC (stage I-IV, n = 159) using formalin-fixed paraffin-embedded tissue samples. In situ hybridization (ISH) using an miR-148a-specific probe was also performed. To further confirm the direct effect of miR-148a on matrix metalloproteinase (MMP)7 expression in CRC, MTT and cell invasion assays using HT29 and WiDr cells were performed. <b><i>Results:</i></b> miR-148a expression was found to be clearly downregulated in high-grade adenoma compared to low-grade adenoma on both qRT-PCR and ISH analysis. Downregulation of miR-148a expression was significantly correlated with advanced clinicopathological features and was an independent prognostic classifier in patients with stage III CRC. In CRC cells and tissues, miR-148a expression was inversely correlated with the expression of MMP7. <b><i>Conclusion:</i></b> We showed the collaborative participation of miR-148a and MMP7 in CRC cell invasion. These results also demonstrate that the downregulation of miR-148a expression promotes CRC progression, especially carcinogenesis and cancer cell invasion. MicroRNAs;Colorectal cancer;miR-148a;Matrix metalloproteinase 7 2015-09-22
    https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Significance_of_miR-148a_in_Colorectal_Neoplasia_Downregulation_of_miR-148a_Contributes_to_the_Carcinogenesis_and_Cell_Invasion_of_Colorectal_Cancer/5128558
10.6084/m9.figshare.5128558.v1