%0 Generic %A O.-C., Back %A H.-S., Jang %A J.-S., Lee %A D.-E., Kim %A Y., Lee %A E.-S., Park %A I.S., Kim %D 2015 %T Supplementary Material for: The Protective Effect of INH2BP, a Novel PARP Inhibitor 5-Iodo-6-Amino-1,2-Benzopyrone, Against Hydrogen Peroxide-Induced Apoptosis Through ERK and p38 MAPK in H9c2 Cells %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_The_Protective_Effect_of_INH_sub_2_sub_BP_a_Novel_PARP_Inhibitor_5-Iodo-6-Amino-1_2-Benzopyrone_Against_Hydrogen_Peroxide-Induced_Apoptosis_Through_ERK_and_p38_MAPK_in_H9c2_Cells/5128738 %R 10.6084/m9.figshare.5128738.v1 %2 https://karger.figshare.com/ndownloader/files/8717059 %K Apoptosis %K INH2BP %K Reactive oxygen species %X INH2BP (5-iodo-6-amino-1,2-benzopyrone), a poly-ADP ribose polymerase inhibitor, has been shown to possess anti-cancer, anti-viral, and anti-inflammation properties. The aim of this study was to investigate the protective effect of INH2BP against oxidative stress-induced apoptosis in H9c2 cardiomyoblast cells. While the treatment of H9c2 cardiomyoblasts cells with hydrogen peroxide (H2O2) caused a loss of cell viability and an increase in the number of apoptotic cells, INH2BP significantly protected the cells against H2O2-induced cell death without any cytotoxicity. Our data also shows that INH2BP significantly scavenged intracellular reactive oxygen species (ROS), and markedly enhanced the expression of antioxidant enzymes such as Mn-SOD (superoxide) and Cu/Zn-SOD, and heme oxygenase-1, which was accompanied by the concomitant activation of extracellular regulated kinase 1/2 (ERK1/2) and p38 mitogen-activated protein kinase (MAPK) phosphorylation in H9c2 cells. The effects of INH2BP on ERK1/2 and p38 MAPK phosphorylation were abrogated by PD98059, an ERK1/2 inhibitor, and SB203580, a p38 inhibitor. In addition, inhibition of ERK1/2 and p38 MAPK by these inhibitors significantly attenuated INH2BP-mediated H9c2 viability as well as cleaved caspases-3, Bax, and Bcl-2 activation. Taken together, these results demonstrate that INH2BP prevents H2O2-induced apoptosis in H9c2 cells by reducing the production of intracellular ROS, regulating apoptotic-related proteins, and the activation of the ERK1/2 and p38 MAPK. %I Karger Publishers