V., Leone C., Langella F., Esposito De Martino M. M., Decaussin-Petrucci G., Chiappetta A., Bianco A., Fusco Supplementary Material for: miR-130b-3p Upregulation Contributes to the Development of Thyroid Adenomas Targeting <b><i>CCDC6</i></b> Gene We have previously studied the function of microRNAs (miRNAs) in thyroid cells using the differentiated rat thyroid PC Cl 3 cells that need thyrotropin (TSH) for their growth. The miRNA expression profile examination allowed the detection of a set of miRNAs downregulated and upregulated by TSH. Here, we first demonstrated that upregulation of miR-130b-3p occurs through a protein kinase A-cAMP-responsive element binding protein (CREB)-dependent mechanism. Then, we analyzed its expression in human thyroid follicular adenomas, where a constitutive CREB activation is frequently present. miR-130b-3p results in upregulation with a high fold-change in most thyroid follicular adenomas. Then, we identified <i>CCDC6</i>, coding for a protein that interacts with CREB1 leading to the transcriptional repression of CREB1 target genes, as a target of this miRNA. The targeting of CCDC6 by miR-130b-3p likely accounts for the mechanism by which its upregulation contributes to the development of thyroid adenomas increasing CREB1 activity. Adenoma;Cell cycle;MicroRNA;Thyroid 2015-11-21
    https://karger.figshare.com/articles/dataset/Supplementary_Material_for_miR-130b-3p_Upregulation_Contributes_to_the_Development_of_Thyroid_Adenomas_Targeting_b_i_CCDC6_i_b_Gene/5128909
10.6084/m9.figshare.5128909.v1