10.6084/m9.figshare.5129392.v1 Chandler J.C. Chandler J.C. Aizen J. Aizen J. Elizur A. Elizur A. Battaglene S.C. Battaglene S.C. Ventura T. Ventura T. Supplementary Material for: Male Sexual Development and the Androgenic Gland: Novel Insights through the de novo Assembled Transcriptome of the Eastern Spiny Lobster,<b><i> Sagmariasus verreauxi</i></b> Karger Publishers 2016 Androgenic gland Decapod DM-domain Dmrt Insulin-like androgenic gland hormone Male-biased expression Sexual development 2016-03-01 00:00:00 Dataset https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Male_Sexual_Development_and_the_Androgenic_Gland_Novel_Insights_through_the_de_novo_Assembled_Transcriptome_of_the_Eastern_Spiny_Lobster_b_i_Sagmariasus_verreauxi_i_b_/5129392 The Eastern spiny lobster, <i>Sagmariasus verreauxi</i>, is commercially important in fisheries, with growing aquaculture potential, driving an interest to better understand male sexual differentiation. Amongst the Decapoda<i>, </i>the androgenic gland (AG) and the insulin-like androgenic gland hormone (IAG) have a well-defined function in male sexual differentiation. However, IAG is not a sex determinant and therefore must be considered as part of a broader, integrated pathway. This work uses a transcriptomic, multi-tissue approach to provide an integrated description of male-biased expression as mediated through the AG. Transcriptomic analyses demonstrate that IAG expression is stage- and eyestalk-regulated (low in immature, high in mature and 6-times higher in hypertrophied glands), with IAG being the predominant AG-specific factor. The low expression of this key factor in immature males suggests the involvement of other tissues in male sexual differentiation. Across tissues, the gonad (87.8%) and antennal gland (73.5%) show the highest male-biased differential expression of transcripts and also express 4 sex-determination regulators, known as <i>Dmrts</i>, with broader expression of<i> Sv-Sxl </i>and <i>Sv-TRA-2.</i> In order to better understand male sexual differentiation, tissues other than the AG must also be considered. This research highlights the gonad and antennal gland as showing significant male-biased expression patterns, including the <i>Sv-Dmrts</i>.