Supplementary Material for: Antimelanogenic Efficacy of Melasolv (3,4,5-Trimethoxycinnamate Thymol Ester) in Melanocytes and Three-Dimensional Human Skin Equivalent Lee J.H. Lee E.-S. Bae I.-H. Hwang J.-A. Kim S.-H. Kim D.-Y. Park N.-H. Rho H.S. Kim Y.J. Oh S.-G. Lee C.S. 10.6084/m9.figshare.5155417.v1 https://karger.figshare.com/articles/media/Supplementary_Material_for_Antimelanogenic_Efficacy_of_Melasolv_3_4_5-Trimethoxycinnamate_Thymol_Ester_in_Melanocytes_and_Three-Dimensional_Human_Skin_Equivalent/5155417 <strong><em>Background/Aims:</em></strong> Excessive melanogenesis often causes unaesthetic hyperpigmentation. In a previous report, our group introduced a newly synthesized depigmentary agent, Melasolv™ (3,4,5-trimethoxycinnamate thymol ester). In this study, we demonstrated the significant whitening efficacy of Melasolv using various melanocytes and human skin equivalents as in vitro experimental systems. <b><i>Methods:</i></b> The depigmentary effect of Melasolv was tested in melan-a cells (immortalized normal murine melanocytes), α-melanocyte-stimulating hormone (α-MSH)-stimulated B16 murine melanoma cells, primary normal human melanocytes (NHMs), and human skin equivalent (MelanoDerm). The whitening efficacy of Melasolv was further demonstrated by photography, time-lapse microscopy, Fontana-Masson (F&M) staining, and 2-photon microscopy. <b><i>Results:</i></b> Melasolv significantly inhibited melanogenesis in the melan-a and α-MSH-stimulated B16 cells. In human systems, Melasolv also clearly showed a whitening effect in NHMs and human skin equivalent, reflecting a decrease in melanin content. F&M staining and 2-photon microscopy revealed that Melasolv suppressed melanin transfer into multiple epidermal layers from melanocytes as well as melanin synthesis in human skin equivalent. <b><i>Conclusion:</i></b> Our study showed that Melasolv clearly exerts a whitening effect on various melanocytes and human skin equivalent. These results suggest the possibility that Melasolv can be used as a depigmentary agent to treat pigmentary disorders as well as an active ingredient in cosmetics to increase whitening efficacy. 2017-06-29 12:35:29 Melasolv Melanogenesis Depigmentary agent Three-dimensional human skin equivalent