%0 Generic %A Y., Li %A C.-p., Gan %A S., Zhang %A X.-k., Zhou %A X.-f., Li %A Y.-q., Wei %A J.-l., Yang %A M., Wu %D 2017 %T Erratum: FIP200 is Involved in Murine Pseudomonas Infection by Regulating HMGB1 Intracellular Translocation %U https://karger.figshare.com/articles/dataset/Erratum_FIP200_is_Involved_in_Murine_b_i_Pseudomonas_i_b_Infection_by_Regulating_HMGB1_Intracellular_Translocation/5241664 %R 10.6084/m9.figshare.5241664.v1 %2 https://karger.figshare.com/ndownloader/files/8956363 %K FIP200 %K HMGB1 acetylation %K Acute lung injury %K Pulmonary infection %X Background: FIP200, a critical autophagy initiating protein, can participate in numerous cellular functions including cancer development; however, its functional role in P. aeruginosa infection of alveolar macrophages is unknown. Methods: To investigate the role of FIP200 in host defense, we transfected murine alveolar macrophage MH-S cells with FIP200 siRNA. Having confirmed that FIP200 knockdown inhibited PAO1-induced autophagosme formation, we sought to characterize the underlying signaling pathways by immunoblotting. Further, we used fip200 KO mice to study the effects of fip200 deficiency on HMGB1 translocation. Results: We showed that Pseudomonas PAO1 strain infection facilitated autophagosome formation, whereas knockdown of FIP200 inhibited autophagosome formation and HMGB1 expression in MH-S cells. Silencing FIP200 impaired the translocation of HMGB1 to cytosol of MH-S cells and almost abolished acetylation of HMGB1 during PAO1 infection. In contrast, FIP200 overexpression facilitated the cytosol translocation of HMGB1 from nuclei and increased acetylation of HMGB1 in PAO1-infected MH-S cells. Importantly, expression and acetylation of HMGB1 were also significantly down-regulated in fip200 KO mice following PAO1 infection. Conclusions: Collectively, these findings elucidate that FIP200 may regulate expression and translocation of HMGB1 during PAO1 infection, which may indicate novel therapeutic targets to control pulmonary infection. %I Karger Publishers