%0 Generic
%A Y., Li
%A C.-p., Gan
%A S., Zhang
%A X.-k., Zhou
%A X.-f., Li
%A Y.-q., Wei
%A J.-l., Yang
%A M., Wu
%D 2017
%T Erratum: FIP200 is Involved in Murine Pseudomonas Infection by Regulating HMGB1 Intracellular Translocation
%U https://karger.figshare.com/articles/dataset/Erratum_FIP200_is_Involved_in_Murine_b_i_Pseudomonas_i_b_Infection_by_Regulating_HMGB1_Intracellular_Translocation/5241664
%R 10.6084/m9.figshare.5241664.v1
%2 https://karger.figshare.com/ndownloader/files/8956363
%K FIP200
%K HMGB1 acetylation
%K Acute lung injury
%K Pulmonary infection
%X Background: FIP200, a critical autophagy initiating protein, can participate in numerous cellular functions including cancer development; however, its functional role in P. aeruginosa infection of alveolar macrophages is unknown. Methods: To investigate the role of FIP200 in host defense, we transfected murine alveolar macrophage MH-S cells with FIP200 siRNA. Having confirmed that FIP200 knockdown inhibited PAO1-induced autophagosme formation, we sought to characterize the underlying signaling pathways by immunoblotting. Further, we used fip200 KO mice to study the effects of fip200 deficiency on HMGB1 translocation. Results: We showed that Pseudomonas PAO1 strain infection facilitated autophagosome formation, whereas knockdown of FIP200 inhibited autophagosome formation and HMGB1 expression in MH-S cells. Silencing FIP200 impaired the translocation of HMGB1 to cytosol of MH-S cells and almost abolished acetylation of HMGB1 during PAO1 infection. In contrast, FIP200 overexpression facilitated the cytosol translocation of HMGB1 from nuclei and increased acetylation of HMGB1 in PAO1-infected MH-S cells. Importantly, expression and acetylation of HMGB1 were also significantly down-regulated in fip200 KO mice following PAO1 infection. Conclusions: Collectively, these findings elucidate that FIP200 may regulate expression and translocation of HMGB1 during PAO1 infection, which may indicate novel therapeutic targets to control pulmonary infection.
%I Karger Publishers