Supplementary Material for: Autologous Adipose Tissue Graft to Treat Hypertensive Leg Ulcer: A Pilot Study M.Chopinaud D.Labbé C.Creveuil M.Marc H.Bénateau B.Mourgeon E.Chopinaud A.Veyssière A.Dompmartin 2017 <p><b><i>Background:</i></b> Adipose tissue is an abundant source of mesenchymal stem cells, called adipose-derived stem cells, which have anti-inflammatory and trophic effects to enhance overall tissue repair. <b><i>Objective:</i></b> To determine the healing effects of autologous adipose tissue graft on hypertensive leg ulcers. <b><i>Methods:</i></b> Prospective pilot study in 1 French dermatology department. Ten patients with a hypertensive leg ulcer were enrolled from April 2013 to June 2015. The primary end point was the wound closure rate at each follow-up visit. The secondary end points were wound characteristics, pain assessment and adverse events. <b><i>Results:</i></b> One patient, the only smoker, was lost to follow-up at month 5 (M5). For the 9 non-smokers, wound surfaces constantly and significantly decreased: the median wound closure rate was 73.2% at M3 and 93.1% at M6, <i>p</i> < 0.001. The median wound closure rate of the 10 patients was 63.2% at M3, <i>p</i> < 0.001. Percentages of fibrin and necrosis decreased, granulation tissue increased significantly. Pain rapidly and significantly decreased. No recurrence or adverse event was observed. <b><i>Conclusion:</i></b> Our data suggest that autologous adipose tissue grafting induces pain relief and promotes wound healing with a good skin quality. Large prospective controlled randomized studies are required to confirm these results.</p>