Supplementary Material for: Tissue Engineering of Vein Valves Based on Decellularized Natural Matrices
Mogaldea A.
Goecke T.
Theodoridis K.
Haverich A.
Cebotari S.
Hilfiker A.
10.6084/m9.figshare.5277805.v1
https://karger.figshare.com/articles/journal_contribution/Supplementary_Material_for_Tissue_Engineering_of_Vein_Valves_Based_on_Decellularized_Natural_Matrices/5277805
<p>Valvular repair or transplantation, designed to restore the venous
valve function of the legs, has been proposed as treatment in chronic
venous insufficiency. Available grafts or surgeries have provided
limited durability so far. Generating venous valve substitutes by means
of tissue engineering could be a solution. We generated decellularized
jugular ovine vein conduits containing valves (oVVC) after reseeding
with ovine endothelial cells differentiated from peripheral
blood-derived endothelial cells (oPBEC), cultivated in vitro
corresponding to the circulatory situation in the lower leg at rest and
under exertion<i>. </i>oVVC were decellularized by detergent treatment.
GFP-labeled oPBEC were seeded onto the luminal side of the
decellularized oVVC and cultivated under static-rotational conditions
for 6 h (group I) and 12 h (group II), respectively. Reseeded matrices
of group I were exposed to continuous low flow conditions (“leg at
rest”). The tissues of group II were exposed to a gradually increasing
flow (“leg under effort”). After 5 days, the grafts of group I revealed a
uniform luminal endothelial cell coverage of the examined areas of the
venous walls and adjacent venous valve leaflets. In group II, the cell
coverage on luminal areas of the venous wall parts was found to be
nearly complete. The endothelial cell coverage of adjacent venous valve
leaflets was revealed to be less dense and confluent. Endothelial cells
cultured on acellular vein tissues of both groups were distinctly
orientated uniformly in the flow direction, clearly creating a stable
and flow-orientated layer. Thus, an endothelium could successfully be
reestablished on the luminal surface of a decellularized venous valve by
seeding peripheral blood endothelial cells and culturing under
different conditions.</p>
2017-08-04 10:43:54
Chronic venous insufficiency
Ovine peripheral blood-derived endothelial cells
Ovine vein conduits
Vein valves