%0 Journal Article %A O., Ács %A B., Péterfia %A P., Hollósi %A A., Luczay %A D., Török %A A., Szabó %D 2017 %T Supplementary Material for: Methylation Status of CYP27B1 and IGF2 Correlate to BMI SDS in Children with Obesity %U https://karger.figshare.com/articles/journal_contribution/Supplementary_Material_for_Methylation_Status_of_CYP27B1_and_IGF2_Correlate_to_BMI_SDS_in_Children_with_Obesity/5285323 %R 10.6084/m9.figshare.5285323.v1 %2 https://karger.figshare.com/ndownloader/files/9051697 %K DNA methylation %K Childhood obesity %K Insulin-like growth factor 2 %K Vitamin D %X

Objective: Worldwide increasing childhood obesity is due to interactions between environmental and genetic factors, linked together by epigenetic mechanisms such as DNA methylation. Methods: 82 obese children (>95th BMI percentile , age: 3-18 years) were included. Anthropometric data, metabolic parameters, 25-OH vitamin D (25OHD), and pubertal status were recorded, 24-hour blood pressure monitoring was performed. BMI standard deviation score (SDS) was calculated. Using candidate gene approach, obesity- (insulin-like growth factor 2 (IGF2), proopiomelanocortin (POMC)) and vitamin D metabolism-related genes (1-alfa-hydroxylase (CYP27B1), VDR) regulated by DNA methylation were selected. After isolating DNA from peripheral blood, bisulfite conversion, bisulfite specific polymerase chain reaction (BS-PCR), and pyrosequencing were carried out. Results: No significant correlation between 25-OHD and metabolic parameters and DNA methylation status, but a tendency of positive correlation between VDR methylation status and 25-OHD (r = 0.2053,p = 0.066) were observed. Significant positive correlations between BMI SDS and CYP27B1 hypermethylation (r = 0.2371,p = 0.0342) and a significant negative correlation between IGF2 hypomethylation and BMI SDS (r = -0.305,p = 0.0059) were found. Conclusions Rate of obesity shows correlation with DNA methylation. Hypomethylation of IGF2 and hypermethylation of CYP27B1 genes might positively influence the rate of BMI observed in obese children.

%I Karger Publishers