10.6084/m9.figshare.5311120.v1
Xie Z.
Xie
Z.
Dong Z.
Dong
Z.
Zhu P.
Zhu
P.
Zhang L.
Zhang
L.
Chen X.
Chen
X.
Dong C.
Dong
C.
Supplementary Material for: Antibiotic Susceptibility and Molecular Characterization of Escherichia coli O157 Isolates from Urinary Tract Infections
Karger Publishers
2017
Escherichia coli O157
Urinary tract infections
Antibiotic resistance
Enterobacterial repetitive intergenic consensus sequence PCR
Multilocus sequence typing
2017-08-15 09:18:06
Dataset
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Antibiotic_Susceptibility_and_Molecular_Characterization_of_Escherichia_coli_O157_Isolates_from_Urinary_Tract_Infections/5311120
<p><b><i>Background:</i></b><i>Escherichia coli O157</i> is an important
food-borne pathogen that can cause diarrhoea, hemorrhagic colitis, and
hemolytic uraemic syndrome. Enterobacterial repetitive intergenic
consensus sequence PCR (ERIC-PCR) and multilocus sequence typing (MLST)
are good methods for molecular typing and the extensive use of
antibiotics is a contributing factor to the increasing incidence of
antimicrobial-resistant for these strains. <b><i>Objectives:</i></b> The aim of this study was to determine the prevalence, antimicrobial resistance, and genetic diversity of <i>E. coli O157</i> based on the prevalence of urinary tract infection (UTI) in Hubei, China. <b><i>Results:</i></b> We obtained 23 (8.07%) <i>E. coli O157</i>
isolates from 285 UTI patients in Hubei, China. All isolates were
subjected to antibiotic susceptibility analysis, and molecular typing
was performed using ERIC-PCR and MLST. Antimicrobial susceptibility
results indicated that most strains were resistant to penicillin
(95.65%), chloramphenicol (73.91%), and ampicillin (69.57%). All
isolates were discovered to be multiresistant (resistance to more than 3
antibiotics). Genetic variability analysis showed that all of the
isolates were grouped into 4 clusters both by ERIC-PCR and MLST. <b><i>Conclusion:</i></b> Our findings demonstrated the presence of <i>E. coli O157</i> in UTIs, provided insights into the dissemination of antibiotic-resistant strains, and improved our knowledge of <i>E. coli O157</i> risk assessment in UTIs.</p>