10.6084/m9.figshare.5455057.v1
Mathison A.
Mathison
A.
Liebl A.
Liebl
A.
Bharucha J.
Bharucha
J.
Mukhopadhyay D.
Mukhopadhyay
D.
Lomberk G.
Lomberk
G.
Shah V.
Shah
V.
Urrutia R.
Urrutia
R.
Supplementary Material for: Pancreatic Stellate Cell Models for Transcriptional Studies of Desmoplasia-Associated Genes
Karger Publishers
2017
Pancreatic stellate cells
Cancer
Desmoplasia
transforming growth factor-β
Transcription factors
Epigenetics
2017-09-29 09:20:50
Dataset
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Pancreatic_Stellate_Cell_Models_for_Transcriptional_Studies_of_Desmoplasia-Associated_Genes/5455057
<p><i>Background:</i> Pancreatic stellate cells are emerging as key
players in pathophysiopathological processes underlying the development
of pancreatic disease, including pancreatitis and cancer. The cells are
scarce in the pancreas making their isolation time and resource use
consuming. <i>Methods:</i> Therefore, with the ultimate goal of
facilitating mechanistic studies, here we report the isolation,
characterization, and immortalization of stellate cell lines from rat
and mouse origin. <i>Results:</i> These cell lines display morphological
and molecular markers as well as non-tumorigenic characteristics
similar to the frequently used hepatic counterparts. In addition, we
have tested their robustness as a model for transcriptional regulatory
studies. We find that these cells respond well to TGFβ signaling by
triggering a distinct cascade of gene expression, some genes overlap
with the TGFβ response of LX2 cells. These cells express several key
chromatin proteins and epigenetic regulators involved in the regulation
of gene expression, including co-repressors such as Sin3A (short-term
repression), HP1 (long-term repression), as well as CBP/p300
(activation). Furthermore, these cells are well suited for Gal4-based
transcriptional activation and repression assays. <i>Conclusions:</i>
The cell model reported here may therefore help fuel investigations in
the field of signaling, transcription, and perhaps other studies on
similarly exciting cellular processes.</p>