10.6084/m9.figshare.5455129.v1
Samejima K.
Samejima
K.
Nakatani K.
Nakatani
K.
Suzuki D.
Suzuki
D.
Asai O.
Asai
O.
Sakan H.
Sakan
H.
Yoshimoto S.
Yoshimoto
S.
Yamaguchi Y.
Yamaguchi
Y.
Matsui M.
Matsui
M.
Akai Y.
Akai
Y.
Toyoda M.
Toyoda
M.
Iwano M.
Iwano
M.
Saito Y.
Saito
Y.
Supplementary Material for: Clinical Significance of Fibroblast-Specific Protein-1 Expression on Podocytes in Patients with Focal Segmental Glomerulosclerosis
Karger Publishers
2017
Focal segmental glomerulosclerosis
Fibroblast-specific protein 1
Podocyte
2017-09-29 10:28:21
Figure
https://karger.figshare.com/articles/figure/Supplementary_Material_for_Clinical_Significance_of_Fibroblast-Specific_Protein-1_Expression_on_Podocytes_in_Patients_with_Focal_Segmental_Glomerulosclerosis/5455129
<p><i>Backgrounds/Aims:</i> We previously reported that
fibroblast-specific protein 1 (FSP1) is a marker of
epithelial-mesenchymal transition (EMT) in tubulointerstitial fibrosis.
The EMT-like changes observed in podocytes are reportedly associated
with podocyte detachment which may cause focal glomerulosclerosis. <i>Methods:</i>
In cross-sectional studies, we analyzed podocyte expression of FSP1
immunohistochemically using renal biopsy specimens from 31 patients with
focal segmental glomerulosclerosis (FSGS) and 39 patients with minimal
change disease (MCD). We also semiquantitatively analyzed glomerular
expression of FSP1 mRNA using laser capture microdissection and
real-time PCR. <i>Results:</i> We found that FSP1 was localized to podocytes in both FSGS and MCD patients; however, the number of FSP1<sup>+</sup>
podocytes per glomerular profile was significantly higher in patients
with FSGS than in those with MCD, and there was a corresponding
difference in the levels of FSP1 mRNA. FSP1<sup>+</sup> podocyte counts
per glomerular profile in FSGS patients correlated significantly with
the prevalence of glomerulosclerosis and the extent of interstitial
type-I collagen-positive areas. <i>Conclusion:</i> Taken together, these
data suggest that podocyte expression of FSP1 could shed light on the
potential linkage between EMT-like changes, detachment of podocytes from
the glomerular basal membrane and the pathophysiology underlying FSGS.</p>