10.6084/m9.figshare.5468539.v1
Gutiérrez E.
Gutiérrez
E.
Egido J.
Egido
J.
Rubio-Navarro A.
Rubio-Navarro
A.
Buendía I.
Buendía
I.
Blanco Colio L.M.
Blanco Colio
L.M.
Toldos O.
Toldos
O.
Manzarbeitia F.
Manzarbeitia
F.
de Lorenzo A.
de Lorenzo A.
Sanchez R.
Sanchez
R.
Ortiz A.
Ortiz
A.
Praga M.
Praga
M.
Moreno J.A.
Moreno
J.A.
Supplementary Material for: Oxidative Stress, Macrophage Infiltration and CD163 Expression Are Determinants of Long-Term Renal Outcome in Macrohematuria-Induced Acute Kidney Injury of IgA Nephropathy
Karger Publishers
2017
Acute kidney injury
IgA nephropathy
Hematuria
Macrophages
Oxidative stress
2017-10-04 09:54:33
Figure
https://karger.figshare.com/articles/figure/Supplementary_Material_for_Oxidative_Stress_Macrophage_Infiltration_and_CD163_Expression_Are_Determinants_of_Long-Term_Renal_Outcome_in_Macrohematuria-Induced_Acute_Kidney_Injury_of_IgA_Nephropathy/5468539
<p><b><i>Background:</i></b> Macroscopic hematuria (MH) may cause acute
kidney injury (AKI) in IgA nephropathy. Up to 25% of patients with
MH-associated AKI do not recover baseline renal function. Our objective
was to identify subjects at high risk for an adverse renal function. <b><i>Methods:</i></b>
We examined macrophages, oxidative stress markers (NADPH-p22 and HO-1)
and the hemoglobin scavenger receptor (CD163) in renal biopsy specimens
from 33 MH-AKI patients with complete recovery (CR, n = 17) or
incomplete recovery (IR, n = 16) of renal function after 6.72 (range
0.5–21.5) years of follow-up. <b><i>Results:</i></b> CD163-expressing
macrophages, HO-1 and NADPH-p22 expression were located in areas
surrounding tubules with iron deposits and filled with erythrocyte
casts. CD163-positive macrophages score and HO-1- and p22-positive
staining correlated positively with percentage of tubules with
erythrocyte casts and tubular necrosis. Macrophage infiltration,
CD163-positive macrophage score, NADPH-p22- and HO-1-positive staining
areas were significantly greater in IR patients when compared with CR
patients. The CD163-positive macrophage score and oxidative stress
markers (p22 and HO-1) were negatively correlated with renal function
outcome, as determined by estimated glomerular filtration rate (eGFR)
and proteinuria, at the end of the follow-up period. In multivariate
analysis, the CD163-positive macrophage score remained significantly
associated with final eGFR and proteinuria after adjustment by age,
gender, duration of MH, initial eGFR and proteinuria. <b><i>Conclusions:</i></b>
Increased macrophage infiltration, CD163 expression and oxidative
stress are significant prognostic factors for an IR of renal function in
patients with MH-associated AKI. These molecular pathways may be
involved in the renal response to injury and could be useful to improve
diagnosis and therapeutics.</p>