Supplementary Material for: LncRNA HANR Promotes Tumorigenesis and Increase of Chemoresistance in Hepatocellular Carcinoma
Xiao J.
Lv Y.
Jin F.
Liu Y.
Ma Y.
Xiong Y.
Liu L.
Zhang S.
Sun Y.
Tipoe G.L.
Hong A.
Xing F.
Wang X.
10.6084/m9.figshare.5612005.v1
https://karger.figshare.com/articles/journal_contribution/Supplementary_Material_for_LncRNA_HANR_Promotes_Tumorigenesis_and_Increase_of_Chemoresistance_in_Hepatocellular_Carcinoma/5612005
<p><b><i>Background/Aims:</i></b> Hepatocellular carcinoma (HCC) is the
fifth most common cancer in the world and the third leading cause of
cancer-related death. Critical roles for long non-coding RNAs (lncRNAs)
have recently been demonstrated for a variety of cancers, including
hepatocellular carcinoma. However, the effect and mechanism of lncRNAs
in HCC tumorigenesis and chemoresistance have not been extensively
characterized. <b><i>Methods:</i></b> In the current study, we have identified a HCC-expressed lncRNA termed as <i>HANR</i> (HCC associated long non-coding RNA). We identified <i>HANR</i>
by microarray analysis and validated its up-regulated expression by
quantitative PCR. RNA pull-down and pathway analyses were conducted to
evaluate physical and functional interactions with <i>HANR</i>. <i>In vivo</i> experiments were performed to assess tumorigenesis and increase of chemoresistance. In addition, the <i>HANR</i> expression in HCC specimens was detected by FISH. Xenograft and orthotopic mice model was constructed to observe the effect of <i>HANR</i> on tumorigenesis and chemoresistance <i>in vivo</i>. <b><i>Results:</i></b> <i>HANR</i> was demonstrated to be up-regulated in HCC patients and HCC cell lines. Increased <i>HANR</i> expression in HCC predicted short survival of patients. Knock-down of <i>HANR</i>
markedly retarded cell proliferation, suppressed HCC
xenograft/orthotopic tumor growth, induced apoptosis and enhanced
chemosensitivity to doxorubicin, while over-expression of <i>HANR</i> showed the opposite effects. It was found that <i>HANR</i> bind to GSKIP for regulating the phosphorylation of GSK3β in HCC. <b><i>Conclusion:</i></b> Our results demonstrate that <i>HANR</i>
contributes to the development of HCC and is a promising therapeutic
target for chemosensitization of HCC cells to doxorubicin, which may
represent a promising therapeutic target in the future.</p>
2017-11-17 08:55:50
Hepatocellular carcinoma
Long non-coding RNA
Tumorigenesis
Chemoresistance
GSKIP
GSK3β