10.6084/m9.figshare.5705548.v1
Gresnigt M.S.
Gresnigt
M.S.
Becker K.L.
Becker
K.L.
Leenders F.
Leenders
F.
Alonso M.F.
Alonso
M.F.
Wang X.
Wang
X.
Meis J.F.
Meis
J.F.
Bain J.M.
Bain
J.M.
Erwig L.P.
Erwig
L.P.
van de Veerdonk F.L.
van de Veerdonk F.L.
Supplementary Material for: Differential Kinetics of Aspergillus nidulans and Aspergillus fumigatus Phagocytosis
Karger Publishers
2017
Aspergillus fumigatus
Aspergillus nidulans
Phagocytosis
Phagosome acidification
LC3-associated phagocytosis
Chronic granulomatous disease
2017-12-15 11:15:45
Dataset
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Differential_Kinetics_of_Aspergillus_nidulans_and_Aspergillus_fumigatus_Phagocytosis/5705548
<p>Invasive aspergillosis mainly occurs in immunocompromised patients and is commonly caused by <i>Aspergillus fumigatus</i>, while <i>A.</i><i>nidulans</i> is rarely the causative agent. However, in chronic granulomatous disease (CGD) patients, <i>A. nidulans</i> is a frequent cause of invasive aspergillosis and is associated with higher mortality. Immune recognition of <i>A. nidulans </i>was compared to <i>A. fumigatus </i>to offer an insight into why <i>A. nidulans</i>
infections are prevalent in CGD. Live cell imaging with J774A.1
macrophage-like cells and LC3-GFP-mCherry bone marrow-derived
macrophages (BMDMs) revealed that phagocytosis of <i>A. nidulans </i>was slower compared to <i>A. fumigatus</i>.
This difference could be attributed to slower migration of J774A.1
cells and a lower percentage of migrating BMDMs. In addition, delayed
phagosome acidification and LC3-associated phagocytosis was observed
with <i>A. nidulans</i>. Cytokine and oxidative burst measurements in
human peripheral blood mononuclear cells revealed a lower oxidative
burst upon challenge with <i>A. nidulans</i>. In contrast, <i>A. nidulans</i> induced significantly higher concentrations of cytokines. Collectively, our data demonstrate that <i>A. nidulans </i>is phagocytosed and processed at a slower rate compared to <i>A. fumigatus,</i> resulting in reduced fungal killing and increased germination of conidia. This slower rate of <i>A. nidulans</i> clearance may be permissive for overgrowth within certain immune settings.</p>