Supplementary Material for: Differential Kinetics of Aspergillus nidulans and Aspergillus fumigatus Phagocytosis
Gresnigt M.S.
Becker K.L.
Leenders F.
Alonso M.F.
Wang X.
Meis J.F.
Bain J.M.
Erwig L.P.
van de Veerdonk F.L.
10.6084/m9.figshare.5705548.v1
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Differential_Kinetics_of_Aspergillus_nidulans_and_Aspergillus_fumigatus_Phagocytosis/5705548
<p>Invasive aspergillosis mainly occurs in immunocompromised patients and is commonly caused by <i>Aspergillus fumigatus</i>, while <i>A.</i><i>nidulans</i> is rarely the causative agent. However, in chronic granulomatous disease (CGD) patients, <i>A. nidulans</i> is a frequent cause of invasive aspergillosis and is associated with higher mortality. Immune recognition of <i>A. nidulans </i>was compared to <i>A. fumigatus </i>to offer an insight into why <i>A. nidulans</i>
infections are prevalent in CGD. Live cell imaging with J774A.1
macrophage-like cells and LC3-GFP-mCherry bone marrow-derived
macrophages (BMDMs) revealed that phagocytosis of <i>A. nidulans </i>was slower compared to <i>A. fumigatus</i>.
This difference could be attributed to slower migration of J774A.1
cells and a lower percentage of migrating BMDMs. In addition, delayed
phagosome acidification and LC3-associated phagocytosis was observed
with <i>A. nidulans</i>. Cytokine and oxidative burst measurements in
human peripheral blood mononuclear cells revealed a lower oxidative
burst upon challenge with <i>A. nidulans</i>. In contrast, <i>A. nidulans</i> induced significantly higher concentrations of cytokines. Collectively, our data demonstrate that <i>A. nidulans </i>is phagocytosed and processed at a slower rate compared to <i>A. fumigatus,</i> resulting in reduced fungal killing and increased germination of conidia. This slower rate of <i>A. nidulans</i> clearance may be permissive for overgrowth within certain immune settings.</p>
2017-12-15 11:15:45
Aspergillus fumigatus
Aspergillus nidulans
Phagocytosis
Phagosome acidification
LC3-associated phagocytosis
Chronic granulomatous disease