Supplementary Material for: The Safety and Effectiveness of a Novel Annular Keratopigmentation Method: A Case Report F.Ferrari van Haselen R. 2018 <b><i>Background:</i></b> We investigated the safety and effectiveness of a novel aesthetic femtosecond-assisted annular keratopigmentation technique. <b><i>Case Report:</i></b> A 21-year-old female patient in good general and ophthalmological health with the wish to change the colour of her eyes was treated with a femtosecond-assisted annular keratopigmentation technique. Pigment was inserted in a channel in the cornea (external diameter 9.3 mm; internal diameter 5.5 mm) created with a femtosecond laser at a depth of 225 μm. Eight months post-operatively, there were no signs of leakage, diffusion, inflammation or any other detrimental effects on the cornea both objectively and subjectively. <b><i>Conclusion:</i></b> This procedure is a promising safe and effective option for those who want annular keratopigmentation for aesthetic reasons.