Supplementary Material for: Fecal Microbiome and Food Allergy in Pediatric Atopic Dermatitis: A Cross-Sectional Pilot Study
Fieten K.B.
Totté J.E.E.
Levin E.
Reyman M.
Meijer Y.
Knulst A.
Schuren F.
Pasmans S.G.M.A.
10.6084/m9.figshare.5817411.v1
https://karger.figshare.com/articles/journal_contribution/Supplementary_Material_for_Fecal_Microbiome_and_Food_Allergy_in_Pediatric_Atopic_Dermatitis_A_Cross-Sectional_Pilot_Study/5817411
<p><b><i>Background:</i></b> Exposure to microbes may be important in
the development of atopic disease. Atopic diseases have been associated
with specific characteristics of the intestinal microbiome. The link
between intestinal microbiota and food allergy has rarely been studied,
and the gold standard for diagnosing food allergy (double-blind
placebo-controlled food challenge [DBPCFC]) has seldom been used. We
aimed to distinguish fecal microbial signatures for food allergy in
children with atopic dermatitis (AD). <b><i>Methods:</i></b> Pediatric
patients with AD, with and without food allergy, were included in this
cross-sectional observational pilot study. AD was diagnosed according to
the UK Working Party criteria. Food allergy was defined as a positive
DBPCFC or a convincing clinical history, in combination with
sensitization to the relevant food allergen. Fecal samples were analyzed
using 16S rRNA microbial analysis. Microbial signature species,
discriminating between the presence and absence food allergy, were
selected by elastic net regression. <b><i>Results:</i></b> Eighty-two
children with AD (39 girls) with a median age of 2.5 years, and 20 of
whom were diagnosed with food allergy, provided fecal samples. Food
allergy to peanut and cow's milk was the most common. Six bacterial
species from the fecal microbiome were identified, that, when combined,
distinguished between children with and without food allergy: <i>Bifidobacterium breve</i>,<i> Bifidobacterium pseudocatenulatum</i>,<i> Bifidobacterium adolescentis</i>, <i>Escherichia coli</i>,<i> Faecalibacterium prausnitzii</i>, and <i>Akkermansia muciniphila (</i>AUC 0.83, sensitivity 0.77, specificity 0.80). <b><i>Conclusions:</i></b>
In this pilot study, we identified a microbial signature in children
with AD that discriminates between the absence and presence of food
allergy. Future studies are needed to confirm our findings.</p>
2018-01-24 10:17:14
Pediatric atopic dermatitis
Food allergy
Fecal microbiome
Intestinal microbiota
Microbes