%0 Generic %A H., Hamzeiy %A D., Savaş %A C., Tunca %A N.E., Şen %A A., Gündoğdu Eken %A I., Şahbaz %A D., Calini %A C., Tiloca %A N., Ticozzi %A A., Ratti %A V., Silani %A A.N., Başak %D 2018 %T Supplementary Material for: Elevated Global DNA Methylation Is Not Exclusive to Amyotrophic Lateral Sclerosis and Is Also Observed in Spinocerebellar Ataxia Types 1 and 2 %U https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Elevated_Global_DNA_Methylation_Is_Not_Exclusive_to_Amyotrophic_Lateral_Sclerosis_and_Is_Also_Observed_in_Spinocerebellar_Ataxia_Types_1_and_2/5873358 %R 10.6084/m9.figshare.5873358.v1 %2 https://karger.figshare.com/ndownloader/files/10442373 %2 https://karger.figshare.com/ndownloader/files/10442376 %2 https://karger.figshare.com/ndownloader/files/10442379 %K Amyotrophic lateral sclerosis %K Trinucleotide repeat disorder %K Spinocerebellar ataxia %K ELISA %K Global DNA methylation %K 5-Methylcytosine %X Adult-onset neurological disorders are caused and influenced by a multitude of different factors, including epigenetic modifications. Here, using an ELISA kit selected upon careful testing, we investigated global 5-methylcytosine (5-mC) levels in sporadic and familial amyotrophic lateral sclerosis (sALS and fALS), spinocerebellar ataxia types 1 and 2 (SCA1 and SCA2), Huntington’s disease, Friedreich’s ataxia, and myotonic dystrophy type 1. We report a significant elevation in global 5-mC levels of about 2–7% on average for sALS (p < 0.01 [F(1, 243) = 9.159, p = 0.0027]) and various forms of fALS along with SCA1 (p < 0.01 [F(1, 83) = 11.285], p = 0.0012) and SCA2 (p < 0.001 [F(1, 122) = 29.996, p = 0.0001]) when compared to age- and sex-matched healthy controls. C9orf72 expansion carrier ALS patients exhibit the highest global 5-mC levels along with C9orf72 promoter hypermethylation. We failed to measure global 5-hydroxymethylcytosine (5-hmC) levels in blood, probably due to the very low levels of 5-hmC and the limitations of the commercially available ELISA kits. Our results point towards a role for epigenetics modification in ALS, SCA1, and SCA2, and help conclude a dispute on the global 5-mC levels in sALS blood. %I Karger Publishers