10.6084/m9.figshare.5951797.v1
Pfannes E.K.B.
Pfannes
E.K.B.
Weiss L.
Weiss
L.
Hadam S.
Hadam
S.
Gonnet J.
Gonnet
J.
Combardière B.
Combardière
B.
Blume-Peytavi U.
Blume-Peytavi
U.
Vogt A.
Vogt
A.
Supplementary Material for: Physiological and Molecular Effects of in vivo and ex vivo Mild Skin Barrier Disruption
Karger Publishers
2018
Skin barrier
Cyanoacrylate skin surface stripping
Transcutaneous vaccination
Particle penetration
Tape stripping
2018-03-06 10:05:20
Dataset
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Physiological_and_Molecular_Effects_of_in_vivo_and_ex_vivo_Mild_Skin_Barrier_Disruption/5951797
<p>The success of topically applied treatments on skin relies on the
efficacy of skin penetration. In order to increase particle or product
penetration, mild skin barrier disruption methods can be used. We
previously described cyanoacrylate skin surface stripping as an
efficient method to open hair follicles, enhance particle penetration,
and activate Langerhans cells. We conducted ex vivo and in vivo
measurements on human skin to characterize the biological effect and
quantify barrier disruption-related inflammation on a molecular level.
Despite the known immunostimulatory effects, this barrier disruption and
hair follicle opening method was well accepted and did not result in
lasting changes of skin physiological parameters, cytokine production,
or clinical side effects. Only in ex vivo human skin did we find a
discrete increase in IP-10, TGF-β, IL-8, and GM-CSF mRNA. The data
underline the safety profile of this method and demonstrate that the
procedure per se does not cause substantial inflammation or skin damage,
which is also of interest when applied to non-invasive sampling of
biomarkers in clinical trials.</p>