10.6084/m9.figshare.6097616.v1 Lee C. Lee C. Eom Y.A. Eom Y.A. Yang H. Yang H. Jang M. Jang M. Jung S.U. Jung S.U. Park Y.O. Park Y.O. Lee S.E. Lee S.E. Jung H. Jung H. Supplementary Material for: Skin Barrier Restoration and Moisturization Using Horse Oil-Loaded Dissolving Microneedle Patches Karger Publishers 2018 Skin barrier restoration Skin barrier function Moisturization Horse oil Microneedle Dermal therapy Clinical practice 2018-04-05 12:07:57 Dataset https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Skin_Barrier_Restoration_and_Moisturization_Using_Horse_Oil-Loaded_Dissolving_Microneedle_Patches/6097616 <b><i>Background:</i></b> Horse oil (HO) has skin barrier restoration and skin-moisturizing effects. Although cream formulations have been used widely and safely, their limited penetration through the stratum corneum is a major obstacle to maximizing the cosmetic efficacy of HO. Therefore, we aimed to encapsulate HO in a cosmetic dissolving microneedle (DMN) for efficient transdermal delivery. <b><i>Methods:</i></b> To overcome these limitations of skin permeation, HO-loaded DMN (HO-DMN) patches were developed and evaluated for their efficacy and safety using in vitro and clinical studies. <b><i>Results:</i></b> Despite the lipophilic nature of HO, the HO-DMN patches had a sharp shape and uniform array, with an average length and tip diameter of 388.36 ± 16.73 and 38.54 ± 5.29 µm, respectively. The mechanical strength of the HO-DMN patches was sufficient (fracture force of 0.29 ± 0.01 N), and they could successfully penetrate pig skin. During the 4-week clinical evaluation, HO-DMN patches caused significant improvements in skin and dermal density, skin elasticity, and moisturization. Additionally, a brief safety assessment showed that the HO-DMN patches induced negligible adverse events. <b><i>Conclusion:</i></b> The HO-DMNs are efficient, safe, and convenient for wide use in cosmetic applications for skin barrier restoration and moisturization.