C., Lee Y.A., Eom H., Yang M., Jang S.U., Jung Y.O., Park S.E., Lee H., Jung Supplementary Material for: Skin Barrier Restoration and Moisturization Using Horse Oil-Loaded Dissolving Microneedle Patches <b><i>Background:</i></b> Horse oil (HO) has skin barrier restoration and skin-moisturizing effects. Although cream formulations have been used widely and safely, their limited penetration through the stratum corneum is a major obstacle to maximizing the cosmetic efficacy of HO. Therefore, we aimed to encapsulate HO in a cosmetic dissolving microneedle (DMN) for efficient transdermal delivery. <b><i>Methods:</i></b> To overcome these limitations of skin permeation, HO-loaded DMN (HO-DMN) patches were developed and evaluated for their efficacy and safety using in vitro and clinical studies. <b><i>Results:</i></b> Despite the lipophilic nature of HO, the HO-DMN patches had a sharp shape and uniform array, with an average length and tip diameter of 388.36 ± 16.73 and 38.54 ± 5.29 µm, respectively. The mechanical strength of the HO-DMN patches was sufficient (fracture force of 0.29 ± 0.01 N), and they could successfully penetrate pig skin. During the 4-week clinical evaluation, HO-DMN patches caused significant improvements in skin and dermal density, skin elasticity, and moisturization. Additionally, a brief safety assessment showed that the HO-DMN patches induced negligible adverse events. <b><i>Conclusion:</i></b> The HO-DMNs are efficient, safe, and convenient for wide use in cosmetic applications for skin barrier restoration and moisturization. Skin barrier restoration;Skin barrier function;Moisturization;Horse oil;Microneedle;Dermal therapy;Clinical practice 2018-04-05
    https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Skin_Barrier_Restoration_and_Moisturization_Using_Horse_Oil-Loaded_Dissolving_Microneedle_Patches/6097616
10.6084/m9.figshare.6097616.v1