X., Liu T., Zhao X., Bai M., Li J., Ren M., Wang R., Xu S., Zhang H., Li Y., Hu L., Xie Y., Zhang L., Yang C., Yan Supplementary Material for: LOC101930370/MiR-1471 Axis Modulates the Hedgehog Signaling Pathway in Breast Cancer <b><i>Background/Aims:</i></b> Non-coding RNAs (ncRNAs) play vital regulatory roles in many tumors. However, the functional roles of these transcripts responsible for their dysregulation in breast cancer (BC) are not thoroughly understood. <b><i>Methods:</i></b> We examined the expression of microRNA miR-1471 in BC specimens. Online analysis tools predicted that lncRNA LOC101930370 might act as an endogenous ‘sponge’ by competing for miR-1471 binding targets. Luciferase assays were used to prove the interaction of LOC101930370, miR-1471 and SHH. Edu, wound-healing and transwell assays were used to verify the contribution of miR-1471 and LOC101930370 on MCF-7 cells proliferation and metastasis. Gain and loss of function studies were performed to evaluate the relevance of Hedgehog pathway with LOC101930370/miR-1471 regulating axis in MCF-7 cells. <b><i>Results:</i></b> The expression of miR-1471 was markedly downregulated in BC. Inhibition of miR-1471 by LOC101930370 was proved by luciferase assay. Knockdown of LOC101930370 suppressed BC cells progression. MiR-1471 inhibitor resulted in a more aggressive metastasis of MCF-7 cells. Moreover, SHH and Gli-1 expression were significantly suppressed by LOC101930370 knockdown, and upregulated by miR-1471 inhibitor transfection. <b><i>Conclusions:</i></b> Collectively, our study reveals the interaction between LOC101930370 and miR-1471 for the first time. LOC101930370 positively regulates the expression of SHH by sponging miR-1471, which sheds new light on lncRNA-directed diagnostics and therapeutics in BC. MiR-1471;LOC101930370;SHH;Gli-1;Hedgehog pathway;Breast cancer 2018-07-24
    https://karger.figshare.com/articles/dataset/Supplementary_Material_for_LOC101930370_MiR-1471_Axis_Modulates_the_Hedgehog_Signaling_Pathway_in_Breast_Cancer/6854534
10.6084/m9.figshare.6854534.v1