Supplementary Material for: miR-146a Mimics Attenuate Allergic Airway Inflammation by Impacted Group 2 Innate Lymphoid Cells in an Ovalbumin-Induced Asthma Mouse Model Han S. Ma C. Bao L. Lv L. Huang M. 10.6084/m9.figshare.6993545.v1 https://karger.figshare.com/articles/dataset/Supplementary_Material_for_miR-146a_Mimics_Attenuate_Allergic_Airway_Inflammation_by_Impacted_Group_2_Innate_Lymphoid_Cells_in_an_Ovalbumin-Induced_Asthma_Mouse_Model/6993545 <b><i>Background:</i></b> The prevalence of allergic asthma has increased dramatically. Previous studies have found that the microRNA 146a (miR-146a) expression in asthma inhibits cell proliferation and promotes apoptosis of bronchial smooth muscle cells. We aimed to investigate the effect of miR-146a mimics on ovalbumin (OVA)-induced asthma in a mouse model. <b><i>Methods:</i></b> Inflammatory cell infiltration in bronchoalveolar lavage fluid (BALF) was measured by flow cytometry. Levels of OVA-specific immunoglobulin E (IgE) in serum and cytokines in BALF were examined by enzyme-linked immunosorbent assay. For monitoring the airway, the Penh value (% baseline) was measured using a whole-body plethysmograph. <b><i>Results:</i></b> In OVA-induced asthmatic mice, miR-146a significantly suppressed the infiltration of inflammatory cells in BALF and decreased the levels of OVA-specific IgE and T helper 2 cell type cytokines. In addition, miR-146a inhibited the OVA-induced airway hyperresponsiveness and the group 2 innate lymphoid cell responses. Moreover, the effects of miR-146a mimics were dependent on interleukin 33 stimulation. <b><i>Conclusions:</i></b> Our results suggest that miR-146a mimics might serve as an attractive candidate for further preclinical studies as an anti-inflammatory treatment of asthma. 2018-08-22 08:37:59 Airway hyperresponsiveness MicroRNA 146a T helper 2 cell type cytokines Group 2 innate lymphoid cells Interleukin 33